Table 3 Effects of ERK inhibitor on... | American Society of Hematology

Table 3

Effects of ERK inhibitor on cytokine production by TGF-M-DCs and TGF-GM-DCs

	ERK inhibitor
	−	+
Cytokines decreased by ERK inhibitor
CM: TGF-M-DCs, pg/mL
TGFβ	41 ± 13	9 ± 9†
IL-6	20661 ± 2251	9757 ± 3613
CM: TGF-GM-DCs, pg/Ml
TGFβ	27 ± 3	0 ± 0*
IL-6	50305 ± 1282	23300 ± 5558†
IL-1β	86 ± 4	12 ± 6*
SDF-1 α	725 ± 265	176 ± 135
Cytokines increased by ERK inhibitor
CM: TGF-M-DCs, pg/mL
GM-CSF	40 ± 14	155 ± 67
CM: TGF-GM-DCs, pg/mL
GM-CSF	559 ± 67	720 ± 72*
Cytokines unchanged by ERK inhibitor
CM: TGF-M-DCs, pg/mL
VEGF	15 ± 15	33 ± 2
PDGF-BB	151 ± 151	350 ± 60
IL-1β	3 ± 3	4 ± 4
SDF-1α	1141 ± 139	1358 ± 159
CM: TGF-GM-DCs, pg/mL
VEGF	103 ± 4	117 ± 23
PDGF-BB	242 ± 101	363 ± 64

	ERK inhibitor
	−	+
Cytokines decreased by ERK inhibitor
CM: TGF-M-DCs, pg/mL
TGFβ	41 ± 13	9 ± 9†
IL-6	20661 ± 2251	9757 ± 3613
CM: TGF-GM-DCs, pg/Ml
TGFβ	27 ± 3	0 ± 0*
IL-6	50305 ± 1282	23300 ± 5558†
IL-1β	86 ± 4	12 ± 6*
SDF-1 α	725 ± 265	176 ± 135
Cytokines increased by ERK inhibitor
CM: TGF-M-DCs, pg/mL
GM-CSF	40 ± 14	155 ± 67
CM: TGF-GM-DCs, pg/mL
GM-CSF	559 ± 67	720 ± 72*
Cytokines unchanged by ERK inhibitor
CM: TGF-M-DCs, pg/mL
VEGF	15 ± 15	33 ± 2
PDGF-BB	151 ± 151	350 ± 60
IL-1β	3 ± 3	4 ± 4
SDF-1α	1141 ± 139	1358 ± 159
CM: TGF-GM-DCs, pg/mL
VEGF	103 ± 4	117 ± 23
PDGF-BB	242 ± 101	363 ± 64

Cytokine levels were detected on protein array slides in triplicate for each sample treated ± ERK inhibitor U0126. Data represent average cytokine levels from triplicate samples ± SEM from 2 donors.

P < .05 when using 2-tailed paired t test.

†

P < .05 when using 1-tailed paired t test.

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