Expression of surface antigens on EryP/GFP+ cells during embryogenesis
| Category and antigens . | 9.5 dpc . | 12.5 dpc . | 14.5 dpc . |
|---|---|---|---|
| Erythroid | |||
| Ter119 | +++ | +++ | +++ |
| CD71 | +++ | +++ | ++ |
| Hematopoietic | |||
| CD3 | − | − | − |
| CD11b (Mac1) | − | − | − |
| CD19 | − | − | − |
| CD24 | +++ | +++ | +++ |
| CD31 (PECAM1) | − | − | − |
| CD34 | − | − | − |
| CD41 | − | − | − |
| CD45 | − | − | − |
| CD48 | − | − | − |
| CD55 (DAF) | +++ | +++ | +++ |
| CD105 (Endoglin) | − | − | − |
| CD117 (c-Kit) | − | − | − |
| CD147 (Basigin) | +++ | +++ | +++ |
| CD150 | − | − | − |
| Flk1 | − | − | − |
| Gr1 | − | − | − |
| Sca-1 | − | − | − |
| Adhesion molecules | |||
| CD49d (α4 integrin) | − | + | ++ |
| LPAM1 (α4β7 integrin) | − | − | − |
| CD49e (α5 integrin) | ND | + | ++ |
| CD49f (α6 integrin) | − | + | + |
| CD44 | − | + | ++ |
| MECA32 | − | − | − |
| Growth factor receptors | |||
| CD140a (PDGFRα) | − | − | − |
| CD140b (PDGFRβ) | − | − | − |
| CXCR4 | − | − | − |
| Flt4 | − | − | − |
| Category and antigens . | 9.5 dpc . | 12.5 dpc . | 14.5 dpc . |
|---|---|---|---|
| Erythroid | |||
| Ter119 | +++ | +++ | +++ |
| CD71 | +++ | +++ | ++ |
| Hematopoietic | |||
| CD3 | − | − | − |
| CD11b (Mac1) | − | − | − |
| CD19 | − | − | − |
| CD24 | +++ | +++ | +++ |
| CD31 (PECAM1) | − | − | − |
| CD34 | − | − | − |
| CD41 | − | − | − |
| CD45 | − | − | − |
| CD48 | − | − | − |
| CD55 (DAF) | +++ | +++ | +++ |
| CD105 (Endoglin) | − | − | − |
| CD117 (c-Kit) | − | − | − |
| CD147 (Basigin) | +++ | +++ | +++ |
| CD150 | − | − | − |
| Flk1 | − | − | − |
| Gr1 | − | − | − |
| Sca-1 | − | − | − |
| Adhesion molecules | |||
| CD49d (α4 integrin) | − | + | ++ |
| LPAM1 (α4β7 integrin) | − | − | − |
| CD49e (α5 integrin) | ND | + | ++ |
| CD49f (α6 integrin) | − | + | + |
| CD44 | − | + | ++ |
| MECA32 | − | − | − |
| Growth factor receptors | |||
| CD140a (PDGFRα) | − | − | − |
| CD140b (PDGFRβ) | − | − | − |
| CXCR4 | − | − | − |
| Flt4 | − | − | − |
Circulating GFP+ cells were assessed for surfce antigen expression by flow cytometry. Results represent data from at least 3 independent experiments.
— indicates antigen not detected on GFP+ cells; +, < 20% of GFP+ cells expressed antigen; ++, 20%-80% of GFP+ cells expressed antigen; +++, > 80 of GFP+ expressed antigen; ND, not determined; dpc, days after coitus.