Gal-2 and Gal-4 induce PS exposure in MOLT-4 and HL60 cells in a carbohydrate-dependent fashion
| Treatment . | Annexin-V+/PI−, %* . | |
|---|---|---|
| MOLT-4 . | HL60 . | |
| Control | 2 | 4 |
| Gal-2 | 47 | 49 |
| Gal-2 + lactose† | 6 | 8 |
| Gal-2 + sucrose | 47 | 49 |
| Gal-4 | 37 | 68 |
| Gal-4 + TDG‡ | 4 | 10 |
| Gal-4 + sucrose | 39 | 63 |
| Treatment . | Annexin-V+/PI−, %* . | |
|---|---|---|
| MOLT-4 . | HL60 . | |
| Control | 2 | 4 |
| Gal-2 | 47 | 49 |
| Gal-2 + lactose† | 6 | 8 |
| Gal-2 + sucrose | 47 | 49 |
| Gal-4 | 37 | 68 |
| Gal-4 + TDG‡ | 4 | 10 |
| Gal-4 + sucrose | 39 | 63 |
MOLT-4 or HL60 cells were treated with 10 mM Gal-2 or 3 mM Gal-4 for 4 hours followed by detection for PS exposure. The data shown represent averages of duplicate determinations where the SE was less than 10%.
Gal-2 was coincubated with either 20 mM lactose or 20 mM sucrose as indicated.
Gal-4 was coincubated with either 30 mM TDG or 30 mM sucrose as indicated.