Cumulative probability of forces greater than 10 pN and relative areas of the weak, intermediate, and strong force peaks in the rupture force histograms obtained in different experimental conditions
| Experimental conditions . | Knob-holepairs available . | Cumulative probability, % . | Weak peakarea, % . | Intermediatepeak area, % . | Strong peakarea, % . |
|---|---|---|---|---|---|
| desB-Fibrin + D*-γD364H | B:b | 70.2 ± 8.3† | 51 | 38 | 11 |
| desB-Fibrin + (D-γD364H + 2 mM GHRPam) | B:b | 61.3 ± 7.1 | 63 | 28 | 9 |
| desB-Fibrin + (D-γD364H + 5 mM GHRPam) | B:b | 34.5 ± 5.5† | 62 | 28 | 10 |
| desB-Fibrin + (D-γD364H + 10 mM GHRPam) | B:b | 7.2 ± 1.3† | — | — | — |
| desB-Fibrin + D-γD364H after GHRPam washed out | B:b | 67.3 ± 7.3 | 60 | 26 | 14 |
| desB-Fibrin + (D-γD364H + 10 mM GPRPam) | B:b | 51.5 ± 6.2† | 50 | 38 | 12 |
| desB-Fibrin + D | B:b and B:a | 58.3 ± 10.1‡ | 56 | 39 | 5 |
| desB-Fibrin + (D + 2 mM GPRPam) | B:b and B:a | 61.3 ± 8.4 | 56 | 35 | 9 |
| desB-Fibrin + (D + 10 mM GHRPam) | B:b and B:a | 4.4 ± 1.1‡ | — | — | — |
| desB-Fibrin + D-dimer | B:b and B:a | 65.2 ± 9.3 | 72 | 21 | 7 |
| desA-Fibrin + D-γD364H | A:b | 51.3 ± 7.6§ | 64 | 27 | 9 |
| desA-Fibrin + (D-γD364H + 10 mM GHRPam) | A:b | 5.9 ± 1.3§ | — | — | — |
| Experimental conditions . | Knob-holepairs available . | Cumulative probability, % . | Weak peakarea, % . | Intermediatepeak area, % . | Strong peakarea, % . |
|---|---|---|---|---|---|
| desB-Fibrin + D*-γD364H | B:b | 70.2 ± 8.3† | 51 | 38 | 11 |
| desB-Fibrin + (D-γD364H + 2 mM GHRPam) | B:b | 61.3 ± 7.1 | 63 | 28 | 9 |
| desB-Fibrin + (D-γD364H + 5 mM GHRPam) | B:b | 34.5 ± 5.5† | 62 | 28 | 10 |
| desB-Fibrin + (D-γD364H + 10 mM GHRPam) | B:b | 7.2 ± 1.3† | — | — | — |
| desB-Fibrin + D-γD364H after GHRPam washed out | B:b | 67.3 ± 7.3 | 60 | 26 | 14 |
| desB-Fibrin + (D-γD364H + 10 mM GPRPam) | B:b | 51.5 ± 6.2† | 50 | 38 | 12 |
| desB-Fibrin + D | B:b and B:a | 58.3 ± 10.1‡ | 56 | 39 | 5 |
| desB-Fibrin + (D + 2 mM GPRPam) | B:b and B:a | 61.3 ± 8.4 | 56 | 35 | 9 |
| desB-Fibrin + (D + 10 mM GHRPam) | B:b and B:a | 4.4 ± 1.1‡ | — | — | — |
| desB-Fibrin + D-dimer | B:b and B:a | 65.2 ± 9.3 | 72 | 21 | 7 |
| desA-Fibrin + D-γD364H | A:b | 51.3 ± 7.6§ | 64 | 27 | 9 |
| desA-Fibrin + (D-γD364H + 10 mM GHRPam) | A:b | 5.9 ± 1.3§ | — | — | — |
Values are expressed as mean ± SEM. Cumulative probability of forces greater than 10pN was calculated as percentage of total number of counts. Force peaks: weak = 15 to 20 pN; intermediate = 30 to 40 pN; strong = 50 to 60 pN. Relative areas of force peaks were calculated as percentage of summarized peak areas for each peak.
— indicates indeterminable.
Fibrin(ogen) fragment D.
P < .01.
P < .01.
P < .01.