LBH589 up-regulates expression of apoptosis and DNA damage response–related genes
| MOLT-4 cells . | Reh cells . | ||
|---|---|---|---|
| Function/gene . | Log10 fold up-regulation . | Gene . | Log10 fold up-regulation . |
| Apoptosis | |||
| TP73, CASP8 | 2.75-3 | CASP8,CASP10,CASP5 | 1.75-2 |
| FAS, CASP5 | 3-4.9 | TP73,GADD45A | 2-3.9 |
| DNA damage response | |||
| FANCG | 1.25-1.5 | TP73,CHEK2,GADD45G | 1.25-1.5 |
| GADD45A, RBBP8, TREX1, GADD45G, TP73 | 1.5-2.5 | BRCA1,FANCG,TREX1,RBBP8 | 1.5-4.25 |
| MOLT-4 cells . | Reh cells . | ||
|---|---|---|---|
| Function/gene . | Log10 fold up-regulation . | Gene . | Log10 fold up-regulation . |
| Apoptosis | |||
| TP73, CASP8 | 2.75-3 | CASP8,CASP10,CASP5 | 1.75-2 |
| FAS, CASP5 | 3-4.9 | TP73,GADD45A | 2-3.9 |
| DNA damage response | |||
| FANCG | 1.25-1.5 | TP73,CHEK2,GADD45G | 1.25-1.5 |
| GADD45A, RBBP8, TREX1, GADD45G, TP73 | 1.5-2.5 | BRCA1,FANCG,TREX1,RBBP8 | 1.5-4.25 |
Following treatment with 50 nM LBH589 for 24 hours, PCR array analysis was performed on MOLT-4 and Reh cells. The values represent the ratios of treated versus control cells relative gene expression, normalized to the levels of β-actin mRNA.