Cell-surface expression of CD83 on B cells cultured in salt solutions containing different combinations of calcium, magnesium, phenol red, essential amino acids, nonessential amino acids, glutamine, pyruvate, or glucose
| . | % CD83 (SE), n=4 . | CD83 MFI (SE), n=4 . | P* . |
|---|---|---|---|
| PBS no Ca2+/Mg2+ | 2.40 (1.2) | 3.82 (2.6) | — |
| PBS plus Ca2+/Mg2+ | 30.78 (4.7) | 11.00 (6.4) | < .001 |
| HBSS no Ca2+/Mg2+/PR | 7.11 (1.9) | 5.73 (3.1) | < .05 |
| HBSS no Ca2+/Mg2+ plus PR | 9.30 (2.1) | 7.53 (3.2) | < .05 |
| HBSS plus Ca2+/Mg2+/PR | 32.54 (3.9) | 14.99 (5.5) | < .001 |
| PBS no Ca2+/Mg2+ plus EAA | 0.97 (0.3) | 3.69 (1.7) | NS |
| PBS no Ca2+/Mg2+ plus NAA | 2.75 (1.3) | 3.75 (1.8) | NS |
| PBS no Ca2+/Mg2+ plus EAA plus NAA | 3.51 (2.4) | 4.18 (2.1) | NS |
| PBS no Ca2+/Mg2+ plus glutamine (3.5 mM) | 7.44 (2.9) | 5.61 (3.2) | < .05 |
| PBS no Ca2+/Mg2+ plus pyruvate (100 mM) | 9.35 (3.3) | 6.03 (2.6) | < .05 |
| PBS no Ca2+/Mg2+ plus glucose (2.5%) | 6.32 (2.1) | 6.07 (3.1) | < .05 |
| . | % CD83 (SE), n=4 . | CD83 MFI (SE), n=4 . | P* . |
|---|---|---|---|
| PBS no Ca2+/Mg2+ | 2.40 (1.2) | 3.82 (2.6) | — |
| PBS plus Ca2+/Mg2+ | 30.78 (4.7) | 11.00 (6.4) | < .001 |
| HBSS no Ca2+/Mg2+/PR | 7.11 (1.9) | 5.73 (3.1) | < .05 |
| HBSS no Ca2+/Mg2+ plus PR | 9.30 (2.1) | 7.53 (3.2) | < .05 |
| HBSS plus Ca2+/Mg2+/PR | 32.54 (3.9) | 14.99 (5.5) | < .001 |
| PBS no Ca2+/Mg2+ plus EAA | 0.97 (0.3) | 3.69 (1.7) | NS |
| PBS no Ca2+/Mg2+ plus NAA | 2.75 (1.3) | 3.75 (1.8) | NS |
| PBS no Ca2+/Mg2+ plus EAA plus NAA | 3.51 (2.4) | 4.18 (2.1) | NS |
| PBS no Ca2+/Mg2+ plus glutamine (3.5 mM) | 7.44 (2.9) | 5.61 (3.2) | < .05 |
| PBS no Ca2+/Mg2+ plus pyruvate (100 mM) | 9.35 (3.3) | 6.03 (2.6) | < .05 |
| PBS no Ca2+/Mg2+ plus glucose (2.5%) | 6.32 (2.1) | 6.07 (3.1) | < .05 |
SE indicates standard error; MFI, mean fluorescence intensity; PR, phenol red; EAA, essential amino acids; NAA, nonessential amino acids; and —, not applicable.
Statistics calculated based on the percentage CD83 expression of samples compared with ″PBS no Ca2+/Mg2+″.