Summary of VH186.2 sequences from NP-specific IgG+ B cells
| . | wt . | bim−/− . |
|---|---|---|
| Single cell PCR, no. | 24 | 56 |
| Positive PCR, no. | 17 | 41 |
| Positive VH186.2, no. | 14 | 36 |
| Range mutations per VH186.2, no. | 0-8 | 0-13 |
| Sequences with zero mutations, % | 7.1% | 38.9% |
| Average total mutations per VH186.2, no. (SD) | 4.9 (2.3) | 2.6 (3.4) |
| W33L substitution, n/N (%) | 6/14 (42.9%) | 9/36 (25%) |
| CDR1+CDR2, R/S (ratio) | 37/5 (7.4) | 39/5 (7.8) |
| FW1-FW3, R/S (ratio) | 16/10 (1.6) | 33/14 (2.4) |
| . | wt . | bim−/− . |
|---|---|---|
| Single cell PCR, no. | 24 | 56 |
| Positive PCR, no. | 17 | 41 |
| Positive VH186.2, no. | 14 | 36 |
| Range mutations per VH186.2, no. | 0-8 | 0-13 |
| Sequences with zero mutations, % | 7.1% | 38.9% |
| Average total mutations per VH186.2, no. (SD) | 4.9 (2.3) | 2.6 (3.4) |
| W33L substitution, n/N (%) | 6/14 (42.9%) | 9/36 (25%) |
| CDR1+CDR2, R/S (ratio) | 37/5 (7.4) | 39/5 (7.8) |
| FW1-FW3, R/S (ratio) | 16/10 (1.6) | 33/14 (2.4) |
Details of the sequences are summarized comparing wt with bim−/− B-cells.
R/S indicates ratio of replacement to silent mutations in all sequences; CDR, complementary-determining region; FW, framework region.