Table 2 RT-PCR primers and probes Primer... | American Society of Hematology

Table 2

RT-PCR primers and probes

Primer sequences	Tm; MgCl₂; product size, bp
UL138	55°C; 1.5 mM; 333
5′-TGC GCA TGT TTC TGA GCT AC-3′
5′-ACG GGT TTC AAC AGA TCG AC-3′
UL138 DNA probe	55°C; 1.5 mM; 150
5′-GAG CTG TAC GGG GAG TAC GA-3′
5′-AGC TGC ACT GGG AAG ACA CT-3′
UL122/123	55°C; 2.5 mM; 194
5′-ATT CTT CGG CCA ACT CTG GA-3′
5′-ACA CGA TGG AGT CCT CTG CC-3′
UL122/123 DNA probe	55°C; 2 mM; 201
5′-CCC TGA TAA TCC TGA CGA GG-3′
5′-CAT AGT CTG CAG GAA CGT CGT-3′
GAPDH	60°C; 3 mM; 238
5′-GAG TCA ACG GAT TTG GTC GT-3′
5′-TTG ATT TTG GAG GGA TCT CG-3′

Primer sequences	Tm; MgCl₂; product size, bp
UL138	55°C; 1.5 mM; 333
5′-TGC GCA TGT TTC TGA GCT AC-3′
5′-ACG GGT TTC AAC AGA TCG AC-3′
UL138 DNA probe	55°C; 1.5 mM; 150
5′-GAG CTG TAC GGG GAG TAC GA-3′
5′-AGC TGC ACT GGG AAG ACA CT-3′
UL122/123	55°C; 2.5 mM; 194
5′-ATT CTT CGG CCA ACT CTG GA-3′
5′-ACA CGA TGG AGT CCT CTG CC-3′
UL122/123 DNA probe	55°C; 2 mM; 201
5′-CCC TGA TAA TCC TGA CGA GG-3′
5′-CAT AGT CTG CAG GAA CGT CGT-3′
GAPDH	60°C; 3 mM; 238
5′-GAG TCA ACG GAT TTG GTC GT-3′
5′-TTG ATT TTG GAG GGA TCT CG-3′

Primers are paired, with one forward and one reverse for each.

Tm indicates temperature.