Kinetics of NKG2D ligands expression on SEB-activated CD4+ and CD8+ T cells
| Cells . | Day . | % CFSE* . | Mean MFI ± SD (range) . | |||
|---|---|---|---|---|---|---|
| MICA . | ULBP1 . | ULBP2 . | ULBP3 . | |||
| CD4 | 3 | 46 ± 16 | 24 ± 16 (3-50) | 11 ± 10 (3-28) | 5 ± 2 (4-9) | 8 ± 5 (3-15) |
| CD4 | 5 | 70 ± 17 | 17 ± 11 (3-36) | 35 ± 33 (3-90) | 14 ± 10 (3-33) | 9 ± 4 (3-16) |
| CD4 | 7 | 73 ± 19 | 10 ± 9 (3-22) | 20 ± 17 (7-48) | 14 ± 10 (4-27) | 9 ± 1 (9-10) |
| CD8 | 3 | 40 ± 16 | 29 ± 20 (3-62) | 18 ± 16 (6-47) | 6 ± 2 (4-9) | 8 ± 5 (3-19) |
| CD8 | 5 | 64 ± 19 | 20 ± 13 (4-54) | 33 ± 33 (3-109) | 14 ± 11 (5-34) | 11 ± 8 (3-28) |
| CD8 | 7 | 72 ± 18 | 12 ± 5 (6-17) | 37 ± 37 (7-82) | 10 ± 9 (3-26) | 11 ± 7 (3-18) |
| Cells . | Day . | % CFSE* . | Mean MFI ± SD (range) . | |||
|---|---|---|---|---|---|---|
| MICA . | ULBP1 . | ULBP2 . | ULBP3 . | |||
| CD4 | 3 | 46 ± 16 | 24 ± 16 (3-50) | 11 ± 10 (3-28) | 5 ± 2 (4-9) | 8 ± 5 (3-15) |
| CD4 | 5 | 70 ± 17 | 17 ± 11 (3-36) | 35 ± 33 (3-90) | 14 ± 10 (3-33) | 9 ± 4 (3-16) |
| CD4 | 7 | 73 ± 19 | 10 ± 9 (3-22) | 20 ± 17 (7-48) | 14 ± 10 (4-27) | 9 ± 1 (9-10) |
| CD8 | 3 | 40 ± 16 | 29 ± 20 (3-62) | 18 ± 16 (6-47) | 6 ± 2 (4-9) | 8 ± 5 (3-19) |
| CD8 | 5 | 64 ± 19 | 20 ± 13 (4-54) | 33 ± 33 (3-109) | 14 ± 11 (5-34) | 11 ± 8 (3-28) |
| CD8 | 7 | 72 ± 18 | 12 ± 5 (6-17) | 37 ± 37 (7-82) | 10 ± 9 (3-26) | 11 ± 7 (3-18) |
Analysis was performed on SEB-activated T cells as described in the legend of Figure 2.
Mean % ± SD (standard deviation) of dividing cells quantified by the sequential loss of CFSE fluorescence intensity (gate R1 in Figure 1).