Hamster heart survival in BALB/c nude mice receiving various treatments and in C57BL/6 TCRβ−/− mice
| Group . | Recipients . | Treatment . | n . | Graft survival, mean d ± SD . |
|---|---|---|---|---|
| A | BALB/c nude | No | 8 | > 200 ± 67.1* |
| B | BALB/c nude splenectomized | Transfer of xenograft-rejecting C57BL/6 nude serum 2 weeks after transplantation | 4 | Hyperacute rejection† |
| C | BALB/c nude | Transfer of nonirradiated C57BL/6 nude mouse splenic tissue | 8 | 5 of 8 rejected at 6 POD |
| D | BALB/c nude | Transfer of lethally irradiated C57BL/6 nude mouse splenic tissue | 11 | 9 of 11 rejected at 6 POD |
| E | BALB/c nude | Transfer of lethally irradiated BALB/c nude mouse splenic tissue | 5 | No rejection‡ |
| F | BALB/c nude | Transfer of radioresistant C57BL/6 nude mouse splenic cells‡ | 6 | 4 of 6 rejected at 6 POD |
| G | BALB/c nude | Transfer of NK1.1+ cells§ obtained from C57BL/6 nude mice | 6 | 4 of 6 rejected at 5-6 POD∥ |
| H | BALB/c nude | Transfer of NK1.1− cells§ obtained from C57BL/6 nude mice | 4 | No rejection¶ |
| I | BALB/c nude | Transfer of NK1.1+ cells obtained from C57BL/6 IFN-γ−/− mice | 4 | 3 of 4 rejected at 5-6 POD∥ |
| J | C57BL/6 TCRβ−/− | No | 10 | 8 of 10 rejected at 5-7 POD |
| K | BALB/c nude | Transfer of spleen cells obtained from C57BL/6 TCRβ−/− mice | 4 | 3 of 4 rejected at day 6-7 |
| Group . | Recipients . | Treatment . | n . | Graft survival, mean d ± SD . |
|---|---|---|---|---|
| A | BALB/c nude | No | 8 | > 200 ± 67.1* |
| B | BALB/c nude splenectomized | Transfer of xenograft-rejecting C57BL/6 nude serum 2 weeks after transplantation | 4 | Hyperacute rejection† |
| C | BALB/c nude | Transfer of nonirradiated C57BL/6 nude mouse splenic tissue | 8 | 5 of 8 rejected at 6 POD |
| D | BALB/c nude | Transfer of lethally irradiated C57BL/6 nude mouse splenic tissue | 11 | 9 of 11 rejected at 6 POD |
| E | BALB/c nude | Transfer of lethally irradiated BALB/c nude mouse splenic tissue | 5 | No rejection‡ |
| F | BALB/c nude | Transfer of radioresistant C57BL/6 nude mouse splenic cells‡ | 6 | 4 of 6 rejected at 6 POD |
| G | BALB/c nude | Transfer of NK1.1+ cells§ obtained from C57BL/6 nude mice | 6 | 4 of 6 rejected at 5-6 POD∥ |
| H | BALB/c nude | Transfer of NK1.1− cells§ obtained from C57BL/6 nude mice | 4 | No rejection¶ |
| I | BALB/c nude | Transfer of NK1.1+ cells obtained from C57BL/6 IFN-γ−/− mice | 4 | 3 of 4 rejected at 5-6 POD∥ |
| J | C57BL/6 TCRβ−/− | No | 10 | 8 of 10 rejected at 5-7 POD |
| K | BALB/c nude | Transfer of spleen cells obtained from C57BL/6 TCRβ−/− mice | 4 | 3 of 4 rejected at day 6-7 |
POD indicates postoperative day.
Some recipients were sacrificed for analysis later than day 60 after xenoheart transplantation.
Rejection occurred within 1 to 4 hours.
Radioresistant splenic cells obtained from collagenase-treated lethally irradiated C57BL/6 nude mouse spleens (see “Surgical procedures”).
A total of 6 × 106 cells were transferred.
Severe inflammatory lesions occurred in the remaining mice.
Experiments were terminated at 60 days after hamster heart transplantation.
A total of 100 × 106 cells were transferred.