Table 1 Oligonucleotide primers and... | American Society of Hematology

Table 1

Oligonucleotide primers and probes of KIR amplification

Gene	Forward primer, 5′-3′	Reverse primer, 5′-3′	Amplicon size, bp	Taqman MGB probes, 6FAM 5′-3′ MGBNFQ	Primer conc, nM	Probe conc, nM
2DL1*	GCAGCACCATGTCGCTCT	GTCACTGGGAGCTGACAC	355	ATGCTGACGAACAAGAG	200	50
2DL2	GGAGGGGGAGGCCCATGAAT	GTCGGGGGTTACCGGTTTTA	241	CCAAGGTCAACGGAACA	100	100
2DL3	CCACTGAACCAAGCTCCG	CAGGAGACAACTTTGGATCA	351	CTGGTGCTGCAACAA	150	100
2DL4	AAACCTTCGCTTACAGCCCG	CACTGAGTACCTAATCACAG	374	TGCCCAGCATCAAT	150	100
2DL5	CTCTACAACAAAATATTCTGGAAGAGCA	CCGGCTGGGCTGAGAGT	180	TCACAGGTCTATTTGGGAAA	200	100
2DS1*	TCTCCATCA GTCGCATGR	AGGGCCCAGAGGAAAGTT	313	AGGTCTATATGAGAAACCT	300	50
2DS2	TGCACAGAGAGGGGAAGTA	CACGCTCTCTCCTGCCAA	256	GTCATCACAGGTCTATATGA	100	100
2DS3*	TCACTCCCCCTATCAGTTT	GCATCTGTAGGTTCCTCCT	279	GCCCCACGGTTCT	300	200
2DS4*	CTGGCCCTCCCAGGTCA	GGAATGTTCCGTTGATGC	450	AAGTTTAACAACACTTTGCACC	500	50
2DS5	GCTCATGGTCATCAGCATGG	CGACCGATGGAGAAGTTGC	260	CACATGAGGGATTCC	150	100
3DL1 (1278)	GCCTGCAGGGAACAGAACAGCC	AGAGAGGCACCAGATTTGTGGC	365	CTGAAGGCGTGAGTCTT	150	100
3DL1 (ALL)	AGAACAGCCAACAGCGAGG	ATCTGGGCTTAGCATTTGGAAG	171	CCTACAGATACCATCTTGTACA	100	100
3DL2*	CGGTCCCTTGATGCCTGT	GACCACACGCAGGGCAG	368	TGATCACAGGTCTATATGAG	150	50
3DL3	AGGGACCTACAGATGTTGCA	CTCTCTGTGCAGAAGGAAGC	204	CCAGCAACCCTGTGGTGATCAT	300	150
3DS1*	GCACCCAGCAACCCCA	TAGGTCCCTGCAAGGGCAC	256	AATTTCTCCATCGGTTCCATGA	300	100
NKG2A	CTCCAGAGAAGCTCATTGTTGG	CACCAATCCATGAGGATGGTG	329	CGATAGTTGTTATTCCCTCTACA	250	100
NKG2D	ACAGCTGGGAGATGAGTGAATTTC	TGACTTCACCAGTTTAAGTAAATCCTG	421	TAGAGAAAATGCATCTCCA	550	100
NKG2E	GCCTGTGCTTCAAAGAACTCTTCT	CACACTGGTCTGATATAAGTCCACG	231	CAAACATGAGATAAAAGAC	250	50

Gene	Forward primer, 5′-3′	Reverse primer, 5′-3′	Amplicon size, bp	Taqman MGB probes, 6FAM 5′-3′ MGBNFQ	Primer conc, nM	Probe conc, nM
2DL1*	GCAGCACCATGTCGCTCT	GTCACTGGGAGCTGACAC	355	ATGCTGACGAACAAGAG	200	50
2DL2	GGAGGGGGAGGCCCATGAAT	GTCGGGGGTTACCGGTTTTA	241	CCAAGGTCAACGGAACA	100	100
2DL3	CCACTGAACCAAGCTCCG	CAGGAGACAACTTTGGATCA	351	CTGGTGCTGCAACAA	150	100
2DL4	AAACCTTCGCTTACAGCCCG	CACTGAGTACCTAATCACAG	374	TGCCCAGCATCAAT	150	100
2DL5	CTCTACAACAAAATATTCTGGAAGAGCA	CCGGCTGGGCTGAGAGT	180	TCACAGGTCTATTTGGGAAA	200	100
2DS1*	TCTCCATCA GTCGCATGR	AGGGCCCAGAGGAAAGTT	313	AGGTCTATATGAGAAACCT	300	50
2DS2	TGCACAGAGAGGGGAAGTA	CACGCTCTCTCCTGCCAA	256	GTCATCACAGGTCTATATGA	100	100
2DS3*	TCACTCCCCCTATCAGTTT	GCATCTGTAGGTTCCTCCT	279	GCCCCACGGTTCT	300	200
2DS4*	CTGGCCCTCCCAGGTCA	GGAATGTTCCGTTGATGC	450	AAGTTTAACAACACTTTGCACC	500	50
2DS5	GCTCATGGTCATCAGCATGG	CGACCGATGGAGAAGTTGC	260	CACATGAGGGATTCC	150	100
3DL1 (1278)	GCCTGCAGGGAACAGAACAGCC	AGAGAGGCACCAGATTTGTGGC	365	CTGAAGGCGTGAGTCTT	150	100
3DL1 (ALL)	AGAACAGCCAACAGCGAGG	ATCTGGGCTTAGCATTTGGAAG	171	CCTACAGATACCATCTTGTACA	100	100
3DL2*	CGGTCCCTTGATGCCTGT	GACCACACGCAGGGCAG	368	TGATCACAGGTCTATATGAG	150	50
3DL3	AGGGACCTACAGATGTTGCA	CTCTCTGTGCAGAAGGAAGC	204	CCAGCAACCCTGTGGTGATCAT	300	150
3DS1*	GCACCCAGCAACCCCA	TAGGTCCCTGCAAGGGCAC	256	AATTTCTCCATCGGTTCCATGA	300	100
NKG2A	CTCCAGAGAAGCTCATTGTTGG	CACCAATCCATGAGGATGGTG	329	CGATAGTTGTTATTCCCTCTACA	250	100
NKG2D	ACAGCTGGGAGATGAGTGAATTTC	TGACTTCACCAGTTTAAGTAAATCCTG	421	TAGAGAAAATGCATCTCCA	550	100
NKG2E	GCCTGTGCTTCAAAGAACTCTTCT	CACACTGGTCTGATATAAGTCCACG	231	CAAACATGAGATAAAAGAC	250	50

Primers from Uhrberg et al.⁴⁷

conc indicates concentration.