Homing, migration, and CXCR4 expression of marrow or GROβΔ4-mobilized-SKL cells pretreated with AMD3100
| Cells . | % Reduction in homing . | % Reduction in migration to SDF-1α . | Mean fluorescent intensity for CXCR4 . |
|---|---|---|---|
| Marrow SKL cells | 45 ± 6%* | 30 ± 3%* | 13.1 ± 0.4 |
| GROβΔ4 mobilized SKL cells | −2 ± 12% (NS) | 66 ± 6%* | 30.7 ± 8.8 |
| G-CSF mobilized SKL cells | 37 ± 9%* | ND | ND |
| Cells . | % Reduction in homing . | % Reduction in migration to SDF-1α . | Mean fluorescent intensity for CXCR4 . |
|---|---|---|---|
| Marrow SKL cells | 45 ± 6%* | 30 ± 3%* | 13.1 ± 0.4 |
| GROβΔ4 mobilized SKL cells | −2 ± 12% (NS) | 66 ± 6%* | 30.7 ± 8.8 |
| G-CSF mobilized SKL cells | 37 ± 9%* | ND | ND |
CFSE-stained PBMCs or lin− cells were pretreated with 10 μM AMD3100 or PBS at 37°C for 15 minutes, washed, and transplanted into lethally irradiated recipients or subjected to in vitro migration to 100 ng/mL of recombinant murine SDF-1α. After 4 hours, migrated cells and input cells were enumerated and stained for Sca-1 and c-kit to calculate percent migration of SKL cells. Homing was determined as described in “Materials and methods.” Percent reduction of migration or homing was compared with the cells treated with phosphate-buffered saline. Means (± SEM) fluorescence intensity for CXCR4 from 3 independent experiments are shown.
ND indicates not done.
P < .05 compared with phosphate-buffered saline (N = 3).