Homing of KL and SKL cells mobilized by G-CSF or GROβΔ4
| Mobilizer . | G-CSF . | GROβΔ4 . |
|---|---|---|
| KL cells transplanted | 57 927 ± 5473 | 61 349 ± 4766 |
| (38 436-85 256) | (46 087-85 112) | |
| KL cells recovered | 377 ± 101 | 589 ± 121 |
| (137-927) | (253-1225) | |
| KL cells homed to the recipient marrow | 1256 ± 335 | 1964 ± 408 |
| (457-3090) | (844-4084) | |
| % homing of KL cells‡ | 2.5 ± 1.5 | 3.6 ± 0.8 |
| SKL cells transplanted | 8999 ± 3021 | 7615 ± 1153 |
| (2124-10 762) | (3584-12 494) | |
| SKL cells recovered | 128 ± 21 | 235 ± 38* |
| (61-246) | (111-389) | |
| SKL cells homed to the recipient marrow | 426 ± 70 | 783 ± 127* |
| (204-820) | (369-1296) | |
| % homing of SKL cells‡ | 7.1 ± 1.1† | 11.5 ± 1.8*† |
| Mobilizer . | G-CSF . | GROβΔ4 . |
|---|---|---|
| KL cells transplanted | 57 927 ± 5473 | 61 349 ± 4766 |
| (38 436-85 256) | (46 087-85 112) | |
| KL cells recovered | 377 ± 101 | 589 ± 121 |
| (137-927) | (253-1225) | |
| KL cells homed to the recipient marrow | 1256 ± 335 | 1964 ± 408 |
| (457-3090) | (844-4084) | |
| % homing of KL cells‡ | 2.5 ± 1.5 | 3.6 ± 0.8 |
| SKL cells transplanted | 8999 ± 3021 | 7615 ± 1153 |
| (2124-10 762) | (3584-12 494) | |
| SKL cells recovered | 128 ± 21 | 235 ± 38* |
| (61-246) | (111-389) | |
| SKL cells homed to the recipient marrow | 426 ± 70 | 783 ± 127* |
| (204-820) | (369-1296) | |
| % homing of SKL cells‡ | 7.1 ± 1.1† | 11.5 ± 1.8*† |
Mobilized PBMCs were stained with 5 μM 5-carboxyfluorescein diacetate succinimidyl ester (CFSE) (and 6-CFSE) (Molecular Probes, Eugene, OR) for 10 minutes, as described.59,60 Total KL or SKL cells homed to marrow were calculated as (% CFSE+ KL or SKL cells in recipient marrow) × 100−1 × (% CFSE+ cells in recipient marrow) × 100−1 × (total nucleated recipient marrow cells recovered) × (100/30), assuming marrow cells from 2 femurs, 2 tibias, 2 humerus, and the pelvis (total 8 bones) represents 30% of total body marrow.61 Percent homing was determined as (KL or SKL cells homed to the marrow) ÷ (KL or SKL cells transplanted) × 100 in each recipient and the data were averaged.
The absolute numbers (mean ± SEM, with range in parentheses) of KL or SKL cells recovered from 2 femurs, tibias, humerus, and pelvis of the recipients are shown from 3 experiments with 30 donors and 3 recipients /group/experiment. The number of SKL cells was determined by gating Sca-1+ fraction on KL cells in each experiment.
P < .05 compared with G-CSF.
P < .05 compared with KL cells.
Data are calculated as the average of % homing for each of 9 mice.