PCR detection of NeoR, wild-type HSV-tk, and ΔHSV-tk (truncated) transgenes in GMC clones
| Patient; no. of clones . | 6; 136 . | 7; 612 . | 8; 83 . | 9; 45 . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Mo after BMT and GMC infusion . | 70 . | 106 . | 67 . | 76 . | ||||||
| * | * | * | * | * | * | |||||
| NeoR+ | − | + | − | + | − | + | + | − | − | + |
| HSV-tk+ | − | − | − | − | − | − | − | + | − | − |
| ΔHSV-tk+ | − | − | − | − | − | − | + | − | − | − |
| 5′ LTR PCR (W-09) | ND | + | ND | + | − | + | + | + | − | + |
| Total, no. | 124 | 12 | 609 | 3 | 79 | 2 | 1 | 1 | 31 | 14 |
| Patient; no. of clones . | 6; 136 . | 7; 612 . | 8; 83 . | 9; 45 . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Mo after BMT and GMC infusion . | 70 . | 106 . | 67 . | 76 . | ||||||
| * | * | * | * | * | * | |||||
| NeoR+ | − | + | − | + | − | + | + | − | − | + |
| HSV-tk+ | − | − | − | − | − | − | − | + | − | − |
| ΔHSV-tk+ | − | − | − | − | − | − | + | − | − | − |
| 5′ LTR PCR (W-09) | ND | + | ND | + | − | + | + | + | − | + |
| Total, no. | 124 | 12 | 609 | 3 | 79 | 2 | 1 | 1 | 31 | 14 |
ND indicates not done; +, present; and −, absent.
Data in these columns represent gene-modified clones, positive for LTR PCR.