Table 3 Immunophenotype analysis of... | American Society of Hematology

Table 3

Immunophenotype analysis of spleen cells from Egr1-deficient mice presenting with an MPD

Marker	Cell type	Disease-free		MPD
Marker	Cell type	No. 365 WT*	No. 811−/− †	No. 93+/−	No. 97+/−	No. 199+/−	No. 239+/−	No. 99−/−	No. 251−/−
CD45R B cells	Lymphoid	40	69	11	14	6	9	14	7
CD4 T cells	Lymphoid	12	10	2	6	3	4	7	3
CD8 T cells	Lymphoid	5	5	2	2	2	2	4	1
CD71/Ter119 cells	Erythroid	12	1	75	59	57	26	53	66
Gr-1/Mac-1	Granulocyte/monocyte	19	6	2	11	18	52	12	6
F4/80	Macrophage	5	2	4	5	6	7	6	5
Kit	Immature	1	1	1	< 0.5	1	< 0.5	1	1
CD34	Immature	< 0.5	1	< 0.5	< 0.5	< 0.5	< 0.5	< 0.5	< 0.5
Spleen weight, g	NA	0.1	0.1	0.4	0.9	1.5	0.3	0.2	0.7

Marker	Cell type	Disease-free		MPD
Marker	Cell type	No. 365 WT*	No. 811−/− †	No. 93+/−	No. 97+/−	No. 199+/−	No. 239+/−	No. 99−/−	No. 251−/−
CD45R B cells	Lymphoid	40	69	11	14	6	9	14	7
CD4 T cells	Lymphoid	12	10	2	6	3	4	7	3
CD8 T cells	Lymphoid	5	5	2	2	2	2	4	1
CD71/Ter119 cells	Erythroid	12	1	75	59	57	26	53	66
Gr-1/Mac-1	Granulocyte/monocyte	19	6	2	11	18	52	12	6
F4/80	Macrophage	5	2	4	5	6	7	6	5
Kit	Immature	1	1	1	< 0.5	1	< 0.5	1	1
CD34	Immature	< 0.5	1	< 0.5	< 0.5	< 0.5	< 0.5	< 0.5	< 0.5
Spleen weight, g	NA	0.1	0.1	0.4	0.9	1.5	0.3	0.2	0.7

Analysis performed at the end of study. Numbers in all but the last row indicate percentage of cells.

NA indicates not applicable

WT mouse treated with ENU (disease-free 400 days after ENU injection).

Egr1^−/− mouse, untreated (disease-free at 400 days of age).