Freezer stability data for human and mouse hepcidin
. | Nominal hepcidin standard addition, ng/mL . | Average calculated concentration, ng/mL . | Standard deviation, ng/mL . | % RSD . | % RE . | n . |
---|---|---|---|---|---|---|
Human serum | ||||||
Nonspiked | N/A | <1.0 | N/A | N/A | N/A | 6 |
3× freeze-thaw nonspiked | N/A | <1.0 | N/A | N/A | N/A | 6 |
Seven-wk freezer stability nonspiked | N/A | <1.0 | N/A | N/A | N/A | 6 |
Spiked | 5 | 4.6 | 0.74 | 16.0 | −7.3 | 6 |
3× freeze-thaw spiked | 5 | 4.6 | 0.22 | 4.8 | −7.7 | 6 |
Seven-wk freezer stability spiked | 5 | 4.18 | 0.40 | 9.5 | −16.4 | 6 |
Spiked | 50 | 59.4 | 4.23 | 7.1 | 18.9 | 6 |
3× freeze-thaw spiked | 50 | 57.8 | 4.80 | 8.3 | 15.6 | 6 |
Seven-wk freezer stability spiked | 50 | 44.0 | 6.23 | 14.2 | −12.1 | 6 |
Dilutional linearity: 100 × dilution† | 5000 | 5090.0 | 6.00 | 11.8 | 1.7 | 6 |
Mouse serum | ||||||
Nonspiked | N/A | 3.15* | 0.48 | 15.2 | N/A | 6 |
3× freeze-thaw nonspiked | N/A | 3.12 | 0.20 | 6.6 | −1.1 | 6 |
Eight-wk freezer stability nonspiked | N/A | 2.49 | 0.23 | 0.09 | −20.7 | 6 |
Spiked | 20 | 21.10 | 1.69 | 8.0 | −8.9 | 6 |
3× freeze-thaw spiked | 20 | 19.89 | 0.96 | 4.8 | −14.1 | 6 |
Eight-wk freezer stability spiked | 20 | 16.90 | 1.57 | 9.3 | −24.9 | 6 |
Spiked | 200 | 159.15 | 13.60 | 8.5 | −21.7 | 6 |
3× freeze-thaw spiked | 200 | 149.31 | 5.63 | 3.8 | −26.5 | 6 |
Eight-wk freezer stability spiked | 200 | 183.74 | 8.08 | 4.4 | −9.3 | 6 |
Dilutional linearity: 100 × dilution† | 5000 | 4114.0 | 5.34 | 13.0 | −17.8 | 6 |
. | Nominal hepcidin standard addition, ng/mL . | Average calculated concentration, ng/mL . | Standard deviation, ng/mL . | % RSD . | % RE . | n . |
---|---|---|---|---|---|---|
Human serum | ||||||
Nonspiked | N/A | <1.0 | N/A | N/A | N/A | 6 |
3× freeze-thaw nonspiked | N/A | <1.0 | N/A | N/A | N/A | 6 |
Seven-wk freezer stability nonspiked | N/A | <1.0 | N/A | N/A | N/A | 6 |
Spiked | 5 | 4.6 | 0.74 | 16.0 | −7.3 | 6 |
3× freeze-thaw spiked | 5 | 4.6 | 0.22 | 4.8 | −7.7 | 6 |
Seven-wk freezer stability spiked | 5 | 4.18 | 0.40 | 9.5 | −16.4 | 6 |
Spiked | 50 | 59.4 | 4.23 | 7.1 | 18.9 | 6 |
3× freeze-thaw spiked | 50 | 57.8 | 4.80 | 8.3 | 15.6 | 6 |
Seven-wk freezer stability spiked | 50 | 44.0 | 6.23 | 14.2 | −12.1 | 6 |
Dilutional linearity: 100 × dilution† | 5000 | 5090.0 | 6.00 | 11.8 | 1.7 | 6 |
Mouse serum | ||||||
Nonspiked | N/A | 3.15* | 0.48 | 15.2 | N/A | 6 |
3× freeze-thaw nonspiked | N/A | 3.12 | 0.20 | 6.6 | −1.1 | 6 |
Eight-wk freezer stability nonspiked | N/A | 2.49 | 0.23 | 0.09 | −20.7 | 6 |
Spiked | 20 | 21.10 | 1.69 | 8.0 | −8.9 | 6 |
3× freeze-thaw spiked | 20 | 19.89 | 0.96 | 4.8 | −14.1 | 6 |
Eight-wk freezer stability spiked | 20 | 16.90 | 1.57 | 9.3 | −24.9 | 6 |
Spiked | 200 | 159.15 | 13.60 | 8.5 | −21.7 | 6 |
3× freeze-thaw spiked | 200 | 149.31 | 5.63 | 3.8 | −26.5 | 6 |
Eight-wk freezer stability spiked | 200 | 183.74 | 8.08 | 4.4 | −9.3 | 6 |
Dilutional linearity: 100 × dilution† | 5000 | 4114.0 | 5.34 | 13.0 | −17.8 | 6 |