Table 1 Interassay statistics for the... | American Society of Hematology

Table 1

Interassay statistics for the determination of human and mouse hepcidin by LC/MS/MS

	Nominal validation sample concentration, ng/mL
	Human hepcidin							Mouse hepcidin
	1.0	2.0	5.0	10	50	100	500	0.25	0.50	1.0	2.0	5.0	10.0	50	100	500
Mean, ng/mL	0.96	1.81	4.86	9.64	50.56	99.01	490.93	0.28	0.49	0.92	2.04	5.31	10.71	52.85	103.68	512.29
Standard deviation, ng/mL	0.11	0.22	0.53	1.17	6.59	15.19	71.10	0.01	0.01	0.09	0.08	0.66	1.14	8.41	13.52	64.68
% RSD	11.7	12.2	11.0	12.1	13.0	15.3	14.5	3.6	1.2	10.1	4.0	12.4	10.6	15.9	13.0	12.6
% RE	−4.0	−9.3	−2.8	−3.6	1.1	−1.0	−1.8	12.0	−2.7	−8.3	2.2	6.2	7.1	5.7	3.7	2.5
n	18	18	18	18	18	18	18	18	18	18	18	18	18	18	18	18

	Nominal validation sample concentration, ng/mL
	Human hepcidin							Mouse hepcidin
	1.0	2.0	5.0	10	50	100	500	0.25	0.50	1.0	2.0	5.0	10.0	50	100	500
Mean, ng/mL	0.96	1.81	4.86	9.64	50.56	99.01	490.93	0.28	0.49	0.92	2.04	5.31	10.71	52.85	103.68	512.29
Standard deviation, ng/mL	0.11	0.22	0.53	1.17	6.59	15.19	71.10	0.01	0.01	0.09	0.08	0.66	1.14	8.41	13.52	64.68
% RSD	11.7	12.2	11.0	12.1	13.0	15.3	14.5	3.6	1.2	10.1	4.0	12.4	10.6	15.9	13.0	12.6
% RE	−4.0	−9.3	−2.8	−3.6	1.1	−1.0	−1.8	12.0	−2.7	−8.3	2.2	6.2	7.1	5.7	3.7	2.5
n	18	18	18	18	18	18	18	18	18	18	18	18	18	18	18	18

Data were obtained over 3 separate days of analysis. Duplicate curves and replicate (n=6) validation samples were prepared daily at each concentration to determine intra-assay and interassay statistics

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