Interassay statistics for the determination of human and mouse hepcidin by LC/MS/MS
. | Nominal validation sample concentration, ng/mL . | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Human hepcidin . | Mouse hepcidin . | |||||||||||||||
1.0 . | 2.0 . | 5.0 . | 10 . | 50 . | 100 . | 500 . | 0.25 . | 0.50 . | 1.0 . | 2.0 . | 5.0 . | 10.0 . | 50 . | 100 . | 500 . | |
Mean, ng/mL | 0.96 | 1.81 | 4.86 | 9.64 | 50.56 | 99.01 | 490.93 | 0.28 | 0.49 | 0.92 | 2.04 | 5.31 | 10.71 | 52.85 | 103.68 | 512.29 |
Standard deviation, ng/mL | 0.11 | 0.22 | 0.53 | 1.17 | 6.59 | 15.19 | 71.10 | 0.01 | 0.01 | 0.09 | 0.08 | 0.66 | 1.14 | 8.41 | 13.52 | 64.68 |
% RSD | 11.7 | 12.2 | 11.0 | 12.1 | 13.0 | 15.3 | 14.5 | 3.6 | 1.2 | 10.1 | 4.0 | 12.4 | 10.6 | 15.9 | 13.0 | 12.6 |
% RE | −4.0 | −9.3 | −2.8 | −3.6 | 1.1 | −1.0 | −1.8 | 12.0 | −2.7 | −8.3 | 2.2 | 6.2 | 7.1 | 5.7 | 3.7 | 2.5 |
n | 18 | 18 | 18 | 18 | 18 | 18 | 18 | 18 | 18 | 18 | 18 | 18 | 18 | 18 | 18 | 18 |
. | Nominal validation sample concentration, ng/mL . | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Human hepcidin . | Mouse hepcidin . | |||||||||||||||
1.0 . | 2.0 . | 5.0 . | 10 . | 50 . | 100 . | 500 . | 0.25 . | 0.50 . | 1.0 . | 2.0 . | 5.0 . | 10.0 . | 50 . | 100 . | 500 . | |
Mean, ng/mL | 0.96 | 1.81 | 4.86 | 9.64 | 50.56 | 99.01 | 490.93 | 0.28 | 0.49 | 0.92 | 2.04 | 5.31 | 10.71 | 52.85 | 103.68 | 512.29 |
Standard deviation, ng/mL | 0.11 | 0.22 | 0.53 | 1.17 | 6.59 | 15.19 | 71.10 | 0.01 | 0.01 | 0.09 | 0.08 | 0.66 | 1.14 | 8.41 | 13.52 | 64.68 |
% RSD | 11.7 | 12.2 | 11.0 | 12.1 | 13.0 | 15.3 | 14.5 | 3.6 | 1.2 | 10.1 | 4.0 | 12.4 | 10.6 | 15.9 | 13.0 | 12.6 |
% RE | −4.0 | −9.3 | −2.8 | −3.6 | 1.1 | −1.0 | −1.8 | 12.0 | −2.7 | −8.3 | 2.2 | 6.2 | 7.1 | 5.7 | 3.7 | 2.5 |
n | 18 | 18 | 18 | 18 | 18 | 18 | 18 | 18 | 18 | 18 | 18 | 18 | 18 | 18 | 18 | 18 |
Data were obtained over 3 separate days of analysis. Duplicate curves and replicate (n=6) validation samples were prepared daily at each concentration to determine intra-assay and interassay statistics