Table 1

Interassay statistics for the determination of human and mouse hepcidin by LC/MS/MS

Nominal validation sample concentration, ng/mL
Human hepcidin
Mouse hepcidin
1.02.05.010501005000.250.501.02.05.010.050100500
Mean, ng/mL 0.96 1.81 4.86 9.64 50.56 99.01 490.93 0.28 0.49 0.92 2.04 5.31 10.71 52.85 103.68 512.29 
Standard deviation, ng/mL 0.11 0.22 0.53 1.17 6.59 15.19 71.10 0.01 0.01 0.09 0.08 0.66 1.14 8.41 13.52 64.68 
% RSD 11.7 12.2 11.0 12.1 13.0 15.3 14.5 3.6 1.2 10.1 4.0 12.4 10.6 15.9 13.0 12.6 
% RE −4.0 −9.3 −2.8 −3.6 1.1 −1.0 −1.8 12.0 −2.7 −8.3 2.2 6.2 7.1 5.7 3.7 2.5 
18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 
Nominal validation sample concentration, ng/mL
Human hepcidin
Mouse hepcidin
1.02.05.010501005000.250.501.02.05.010.050100500
Mean, ng/mL 0.96 1.81 4.86 9.64 50.56 99.01 490.93 0.28 0.49 0.92 2.04 5.31 10.71 52.85 103.68 512.29 
Standard deviation, ng/mL 0.11 0.22 0.53 1.17 6.59 15.19 71.10 0.01 0.01 0.09 0.08 0.66 1.14 8.41 13.52 64.68 
% RSD 11.7 12.2 11.0 12.1 13.0 15.3 14.5 3.6 1.2 10.1 4.0 12.4 10.6 15.9 13.0 12.6 
% RE −4.0 −9.3 −2.8 −3.6 1.1 −1.0 −1.8 12.0 −2.7 −8.3 2.2 6.2 7.1 5.7 3.7 2.5 
18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 

Data were obtained over 3 separate days of analysis. Duplicate curves and replicate (n=6) validation samples were prepared daily at each concentration to determine intra-assay and interassay statistics

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