Plasminogen binding was measured by incubating both RAW 264.7 and J774A.1 cells with Alexa 488–labeled Plg (200 nM) for 60 minutes at 4°C. The cells were washed and FACS analyses were used to measure Plg binding and Plg-R expression. Plg-R expression patterns were measured by reacting the cells with each anti–Plg-R, followed by secondary staining with FITC-conjugated goat anti–rabbit IgG. Mean fluorescence intensities (MFIs) have background MFI values in the presence of EACA for Plg or nonimmune IgG for Plg-R subtracted.