Phenotypic characteristics of BM-derived and sessile KCs following bone marrow transplantation
| . | BM-derived . | Sessile . |
|---|---|---|
| IAb | + | + |
| GR1 | − | − |
| CD115 | + | + |
| CXCR4 | + | + |
| CD40 | (+) | (+) |
| Fcγ R | + | + |
| TLR4R | ++ | ++ |
| CD14 | (+) | (+) |
| CD80 (B7–1) | ++ | (+) |
| CD86 (B7–2) | ++ | ++ |
| . | BM-derived . | Sessile . |
|---|---|---|
| IAb | + | + |
| GR1 | − | − |
| CD115 | + | + |
| CXCR4 | + | + |
| CD40 | (+) | (+) |
| Fcγ R | + | + |
| TLR4R | ++ | ++ |
| CD14 | (+) | (+) |
| CD80 (B7–1) | ++ | (+) |
| CD86 (B7–2) | ++ | ++ |
There was no detectable difference in the expression of differentiation markers, homing receptors, or endotoxin receptors on the 2 KC subsets. Although not exclusive, an increased expression of the costimulatory molecule CD80 (B7–1) was noted on BM-derived KCs. Sections were stained with the antibody of interest and costained with F4/80 and either CD45.1 or CD45.2 antibodies.
Incidence of marker presence: −, absent; (+), less than 10%; +, 10-60%; ++, more than 60%; +++, all cells.