Thymocyte gene expression alterations induced by the nuclear translocation of the ERT2-SCL fusion-protein
| Gene . | 1.6 weeks of TAM . | 8 weeks of TAM . | ||
|---|---|---|---|---|
| Fold change . | P . | Fold change . | P . | |
| RALDH2 | 5.34 | .001 | 4.2 | .002 |
| Pre–T-cell receptor α | −2.4 | .22 | −4.3 | .021 |
| RAG2 | −9.1 | .02 | −3.6 | .091 |
| Ephrin B1 | 3.0 | .002 | 2.7 | .026 |
| Gene . | 1.6 weeks of TAM . | 8 weeks of TAM . | ||
|---|---|---|---|---|
| Fold change . | P . | Fold change . | P . | |
| RALDH2 | 5.34 | .001 | 4.2 | .002 |
| Pre–T-cell receptor α | −2.4 | .22 | −4.3 | .021 |
| RAG2 | −9.1 | .02 | −3.6 | .091 |
| Ephrin B1 | 3.0 | .002 | 2.7 | .026 |
mRNA was isolated from thymocytes of lck-ERT2-SCL (1.6 weeks TAM of feed, n = 3; 8 weeks of TAM feed, n = 3) and littermate wild-type control mice (1.6 weeks of TAM feed, n = 4; 8 weeks of TAM feed, n = 4) and used for real-time RT-PCR analysis. Fold change data represent fold increase or decrease of gene expression (mean) imposed by the lck-ERT2-SCL transgene induction.