T-cell reconstituting progenitors in nonirradiated recipients
| Noncultured LSK cells* . | Delta1ext-IgG-cultured LSK cells† . | ||
|---|---|---|---|
| No. of noncultured LSK cells injected . | No. of mice engrafted/total no. of mice injected . | No. of Delta1ext-IgG-cultured cells injected . | No. of mice engrafted/total no. of mice injected . |
| 500 | 1/8 | 105 | 0/4 |
| 1000 | 3/10 | 5 × 105 | 1/4 |
| 10000 | 8/9 | 106 | 5/8 |
| — | — | 107 | 4/4 |
| Noncultured LSK cells* . | Delta1ext-IgG-cultured LSK cells† . | ||
|---|---|---|---|
| No. of noncultured LSK cells injected . | No. of mice engrafted/total no. of mice injected . | No. of Delta1ext-IgG-cultured cells injected . | No. of mice engrafted/total no. of mice injected . |
| 500 | 1/8 | 105 | 0/4 |
| 1000 | 3/10 | 5 × 105 | 1/4 |
| 10000 | 8/9 | 106 | 5/8 |
| — | — | 107 | 4/4 |
Noncultured LSK (Ly5.1) cells and Delta1ext-IgG-cultured cells in limiting doses, along with 1 × 105 syngeneic Ly5.1 BM cells, were injected into nonirradiated Rag−/− mice. Successful T-cell reconstitution after HCT was defined as the presence of at least 0.5% donor CD3+ blood T cells at 5 weeks in nonirradiated mice. The data from several limiting dilution experiments were pooled and analyzed by applying Poisson statistics to the single-hit model.
—indicates no data.
No. of progenitors per 103 LSK cells was 1.3.
No. of progenitors per 103 initial LSK cells was 3.7 × 105.