T-cell reconstituting progenitors in irradiated recipients
| Noncultured LSK cells* . | Delta1ext-IgG-cultured LSK cells† . | ||
|---|---|---|---|
| No. of noncultured LSK cells injected . | No. of mice engrafted/total no. of mice injected . | No. of Delta1ext-IgG-cultured cells injected . | No. of mice engrafted/total no. of mice injected . |
| 10 | 0/6 | 2 × 103 | 0/5 |
| 50 | 0/3 | 2 × 104 | 1/8 |
| 100 | 2/6 | 2 × 105 | 6/9 |
| 500 | 5/7 | 2 × 106 | 7/8 |
| 1000 | 7/8 | — | — |
| Noncultured LSK cells* . | Delta1ext-IgG-cultured LSK cells† . | ||
|---|---|---|---|
| No. of noncultured LSK cells injected . | No. of mice engrafted/total no. of mice injected . | No. of Delta1ext-IgG-cultured cells injected . | No. of mice engrafted/total no. of mice injected . |
| 10 | 0/6 | 2 × 103 | 0/5 |
| 50 | 0/3 | 2 × 104 | 1/8 |
| 100 | 2/6 | 2 × 105 | 6/9 |
| 500 | 5/7 | 2 × 106 | 7/8 |
| 1000 | 7/8 | — | — |
Noncultured LSK (Ly5.1) cells and Delta1ext-IgG-cultured cells along with 1 × 105 syngeneic Ly5.1 BM cells in limiting doses were injected into lethally irradiated Ly5.1-congenic mice. Successful T-cell reconstitution after HCT was defined as the presence of at least 0.5% donor CD3+ blood T cells at 3 weeks. The data from several limiting dilution experiments were pooled and analyzed by applying Poisson statistics to the single-hit model.
—indicates no data.
No. of progenitors per 103 LSK cells was 2.3.
No. of progenitors per 103 initial LSK cells was 1.9 × 109.