B-cell populations in the bone marrow of various mice
| Genotype . | No. of mice . | Bone marrow cells, × 106 . | ||
|---|---|---|---|---|
| B220+IgL− . | B220+IgL+ . | B220+IgL−B220+IgL+ . | ||
| Btk+/+PLCγ2+/+ | 6 | 2.8 ± 0.4 | 1.9 ± 0.2 | 1.5 ± 0.1 |
| Btk−/−PLCγ2+/+ | 6 | 3.2 ± 0.6 | 1.5 ± 0.2 | 2.1 ± 0.1 |
| Btk+/+PLCγ2−/− | 5 | 2.1 ± 0.6 | 0.9 ± 0.2 | 2.3 ± 0.3 |
| Btk−/−PLCγ2−/− | 4 | 1.4 ± 0.4*† | 0.2 ± 0.1‡§ | 7.0 ± 1.4‖¶ |
| Genotype . | No. of mice . | Bone marrow cells, × 106 . | ||
|---|---|---|---|---|
| B220+IgL− . | B220+IgL+ . | B220+IgL−B220+IgL+ . | ||
| Btk+/+PLCγ2+/+ | 6 | 2.8 ± 0.4 | 1.9 ± 0.2 | 1.5 ± 0.1 |
| Btk−/−PLCγ2+/+ | 6 | 3.2 ± 0.6 | 1.5 ± 0.2 | 2.1 ± 0.1 |
| Btk+/+PLCγ2−/− | 5 | 2.1 ± 0.6 | 0.9 ± 0.2 | 2.3 ± 0.3 |
| Btk−/−PLCγ2−/− | 4 | 1.4 ± 0.4*† | 0.2 ± 0.1‡§ | 7.0 ± 1.4‖¶ |
Cells were obtained from 1 femur and tibia of 8- to 12-week-old mice of various indicated genotypes. The absolute numbers of B220+IgL− (Igκ−/λ−) and B220+IgL+ (Igκ+/λ+) cells as well as the ratio of these 2 populations were calculated based on total cell counts and fractions of bone marrow cells as determined by FACS analyses. Statistical significance was established by Student t test.
*P = .002 from Btk−/−.
†P = .001 from PLCγ2−/−.
‡P = .002 from Btk−/−.
§P = .001 from PLCγ2−/−.
‖P = .02 from Btk−/−.
¶P = .002 from PLCγ2−/−.