Table 2 Properties of GR G330A compared... | American Society of Hematology

Table 2

Properties of GR G330A compared with wild-type GR

Property	GR G330A	Wild-type GR
Specific activity, IU/mg	90	205
K_M for NADPH, μM	6.5	7.5
K_M for GSSG, μM	85	65
NADPH oxidase activity vs GR activity, %	< 0.01	< 0.01
Reversible release of FAD	Yes	No
T_1/2 at 37°C*	16 min	> 100 h
T_50% without substrate, °C†	60	85
T_50% with 100 μM NADPH, °C†	33	55

Property	GR G330A	Wild-type GR
Specific activity, IU/mg	90	205
K_M for NADPH, μM	6.5	7.5
K_M for GSSG, μM	85	65
NADPH oxidase activity vs GR activity, %	< 0.01	< 0.01
Reversible release of FAD	Yes	No
T_1/2 at 37°C*	16 min	> 100 h
T_50% without substrate, °C†	60	85
T_50% with 100 μM NADPH, °C†	33	55

Starting with 1 IU GR activity per mL in the presence of 50 μM NADPH at pH 7.3.

Starting with 2.5 IU GR activity per mL at pH 6.9.