Enforced expression of SCL in SCL−/Δ bone marrow cells
| Retrovirus . | No. of colonies* . | |||||
|---|---|---|---|---|---|---|
| G . | GM . | M . | Meg . | Blast . | Mast . | |
| GFP | 14 ± 8 | 11 ± 5 | 7 ± 6 | 0 | 5 ± 5 | 6 ± 2 |
| SCL | 29 ± 12† | 12 ± 5 | 5 ± 3 | 6 ± 1† | 4 ± 1 | 16 ± 10† |
| Retrovirus . | No. of colonies* . | |||||
|---|---|---|---|---|---|---|
| G . | GM . | M . | Meg . | Blast . | Mast . | |
| GFP | 14 ± 8 | 11 ± 5 | 7 ± 6 | 0 | 5 ± 5 | 6 ± 2 |
| SCL | 29 ± 12† | 12 ± 5 | 5 ± 3 | 6 ± 1† | 4 ± 1 | 16 ± 10† |
One-thousand GFP+ sorted cells cultured for 7 days in 0.3% agar with IL-3, SCF, and MGDF.
G indicates granulocyte colonies; GM, granulocyte-macrophage colonies; M, macrophage colonies; and Meg, megakaryocyte colonies.
The numbers of colonies are the mean plus or minus SD from 3 independent infections of SCL-deleted (−/Δ) bone marrow cells.
Statistical comparison with cells infected with the GFP retrovirus; P < .01.