Hematopoietic progenitors in SCL+/D and SCL−/D mice
| Tissue/genotype . | No. of colonies* . | ||||||
|---|---|---|---|---|---|---|---|
| BFU-E† . | G . | GM . | M . | Meg . | Blast . | Mast . | |
| Bone marrow | |||||||
| +/Δ | 10 ± 3 | 14 ± 4 | 31 ± 12 | 18 ± 6 | 8 ± 4 | 3 ± 1 | 4 ± 2 |
| −/Δ | 0‡ | 17 ± 7 | 19 ± 5 | 12 ± 3 | 1 ± 1‡ | 3 ± 1 | 50 ± 13‡ |
| Spleen | |||||||
| +/Δ | 2 ± 1 | 4 ± 1 | 4 ± 3 | 4 ± 3 | 2 ± 1 | 2 ± 1 | 0 |
| −/Δ | 0 | 7 ± 2 | 6 ± 3 | 3 ± 2 | 0 | 4 ± 1 | 66 ± 25‡ |
| Tissue/genotype . | No. of colonies* . | ||||||
|---|---|---|---|---|---|---|---|
| BFU-E† . | G . | GM . | M . | Meg . | Blast . | Mast . | |
| Bone marrow | |||||||
| +/Δ | 10 ± 3 | 14 ± 4 | 31 ± 12 | 18 ± 6 | 8 ± 4 | 3 ± 1 | 4 ± 2 |
| −/Δ | 0‡ | 17 ± 7 | 19 ± 5 | 12 ± 3 | 1 ± 1‡ | 3 ± 1 | 50 ± 13‡ |
| Spleen | |||||||
| +/Δ | 2 ± 1 | 4 ± 1 | 4 ± 3 | 4 ± 3 | 2 ± 1 | 2 ± 1 | 0 |
| −/Δ | 0 | 7 ± 2 | 6 ± 3 | 3 ± 2 | 0 | 4 ± 1 | 66 ± 25‡ |
Twenty-five-thousand cells cultured for 7 days in 0.3% agar with IL-3, SCF, and MGDF or methylcellulose with IL-3, SCF, and Epo.
BFU-E indicates blast-forming unit-erythroid; G, granulocyte colonies; GM, granulocyte-macrophage colonies; M, macrophage colonies; and Meg, megakaryocyte colonies.
The numbers of colonies are the mean plus or minus SD from 4 mice of each genotype: SCL heterozygous (+/Δ,) and SCL deleted (−/Δ).
BFU-Es were grown in methylcellulose, while all other colonies were grown in agar.
Statistical comparison with SCL-heterozygous cells using Student t test; P < .05.