Details of serial hematopoietic stem cell transplantations within 2 families
Patient no. (conditioning/ serotherapy/ GVHD prophylaxis) and donor . | HLA-match (mismatched locus) . | Source . | In vitro T-cell depletion . | Total cells/kg . | CD3+ cells/kg . | CD34+ cells/kg . |
|---|---|---|---|---|---|---|
| X1 (Bu16, Cy200/Campath 1G, d –13 to –9/CSA, MTX) | ||||||
| UD | 9/10 (1C) | BM | Campath 1M | 9.0 × 107 | 1.0 × 105 | — |
| X2 (none/none/none) | ||||||
| Brother | 10/10 | BM | None | 3.5 × 108 | 1.0 × 108 | 5.0 × 106 |
| Y1 | ||||||
| First transplant (Bu8, Flu120, Mel125/ATG, d –15 to –11/CSA) | ||||||
| Father | 7/10 (1B, 1C, 1DR) | PBSCs | Miltenyi | 4.6 × 107 | 2.0 × 105 | 3.6 × 107 |
| Second transplant (Cy150/Campath 1G, d –4 to –1/MP, CSA) | ||||||
| Father | 7/10 (1B, 1C, 1DR) | BM | Campath 1M | 6.0 × 108 | — | 1.9 × 107 |
| Father | 7/10 (1B, 1C, 1DR) | PBSCs | Miltenyi | 2.6 × 107 | 1.1 × 105 | 2.0 × 107 |
| Y2 | ||||||
| First transplant (Flu150, Mel140/Campath 1H, d –8 to –4/CSA) | ||||||
| Brother | 10/10 (before BMT) | BM | None | 2.4 × 108 | 2.8 × 107 | 4.8 × 106 |
| Second transplant (Bu16, Cy200/ATG, d –15 to –11/CSA) | ||||||
| Brother | 10/10 (before BMT) | BM | None | 2.3 × 108 | 2.5 × 107 | 2.8 × 106 |
| Father | 7/10 (1B, 1C, 1DR) | PBSCs | Miltenyi | 2.7 × 107 | 0.8 × 104 | 2.3 × 107 |
Patient no. (conditioning/ serotherapy/ GVHD prophylaxis) and donor . | HLA-match (mismatched locus) . | Source . | In vitro T-cell depletion . | Total cells/kg . | CD3+ cells/kg . | CD34+ cells/kg . |
|---|---|---|---|---|---|---|
| X1 (Bu16, Cy200/Campath 1G, d –13 to –9/CSA, MTX) | ||||||
| UD | 9/10 (1C) | BM | Campath 1M | 9.0 × 107 | 1.0 × 105 | — |
| X2 (none/none/none) | ||||||
| Brother | 10/10 | BM | None | 3.5 × 108 | 1.0 × 108 | 5.0 × 106 |
| Y1 | ||||||
| First transplant (Bu8, Flu120, Mel125/ATG, d –15 to –11/CSA) | ||||||
| Father | 7/10 (1B, 1C, 1DR) | PBSCs | Miltenyi | 4.6 × 107 | 2.0 × 105 | 3.6 × 107 |
| Second transplant (Cy150/Campath 1G, d –4 to –1/MP, CSA) | ||||||
| Father | 7/10 (1B, 1C, 1DR) | BM | Campath 1M | 6.0 × 108 | — | 1.9 × 107 |
| Father | 7/10 (1B, 1C, 1DR) | PBSCs | Miltenyi | 2.6 × 107 | 1.1 × 105 | 2.0 × 107 |
| Y2 | ||||||
| First transplant (Flu150, Mel140/Campath 1H, d –8 to –4/CSA) | ||||||
| Brother | 10/10 (before BMT) | BM | None | 2.4 × 108 | 2.8 × 107 | 4.8 × 106 |
| Second transplant (Bu16, Cy200/ATG, d –15 to –11/CSA) | ||||||
| Brother | 10/10 (before BMT) | BM | None | 2.3 × 108 | 2.5 × 107 | 2.8 × 106 |
| Father | 7/10 (1B, 1C, 1DR) | PBSCs | Miltenyi | 2.7 × 107 | 0.8 × 104 | 2.3 × 107 |
Miltenyi is located in Auburn, CA.
Bu16 indicates busulphan 16 mg/kg; Cy200, cyclophosphamide 200 mg/kg; Campath 1G, Campath 1G 1 mg/kg; CSA, cyclosporin A; MTX, methotrexate; UD, unrelated donor; BM, bone marrow; Bu8, 8 mg/kg busulphan; Flu120, 120 mg/m2 fludarabine; Mel125, 125 mg/m2 melphalan; ATG, 25 mg/kg rabbit antithymocyte globulin; PBSCs, peripheral blood stem cells; Cy150, 150 mg/kg cyclophosphamide; MP, methylprednisolone; Flu150, 150 mg/m2 fludarabine; Mel140, 140 mg/m2 melphalan; and Campath 1H, 1 mg/kg Campath 1H.