Table 1.

Three parameters characterizing thrombin-catalyzed fibrin polymerization


Fibrinogen

Lag period, min

Maximum rate of absorbance change, 10-4/s

Δ absorbance
γ387Ile   3.0 ± 0.2   13.1 ± 0.3   0.382 ± 0.020  
γ387Arg   3.0 ± 0.3   7.7 ± 0.2*  0.343 ± 0.012  
γ387Leu   2.5 ± 0.3   4.3 ± 0.2*  0.247 ± 0.018 
γ387Met   2.0 ± 0.2  5.8 ± 0.2*  0.270 ± 0.015§ 
γ387Ala
 
2.4 ± 0.3
 
6.0 ± 0.4*
 
0.369 ± 0.028
 

Fibrinogen

Lag period, min

Maximum rate of absorbance change, 10-4/s

Δ absorbance
γ387Ile   3.0 ± 0.2   13.1 ± 0.3   0.382 ± 0.020  
γ387Arg   3.0 ± 0.3   7.7 ± 0.2*  0.343 ± 0.012  
γ387Leu   2.5 ± 0.3   4.3 ± 0.2*  0.247 ± 0.018 
γ387Met   2.0 ± 0.2  5.8 ± 0.2*  0.270 ± 0.015§ 
γ387Ala
 
2.4 ± 0.3
 
6.0 ± 0.4*
 
0.369 ± 0.028
 
*

Significantly different from γ387Ile (P < .001).

Significantly different from γ387Ile (P < .01).

Significantly different from γ387Ile (P < .05).

§

Significantly different from γ387Ile (P < .02).

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