Sequences of GST fusion proteins representing a 21–amino acid segment (aa 167–187) of the extracellular loop of CD20
Clone designation . | Sequence of CD20 homolog fused to GST . |
|---|---|
| GST-CD20 | C E P A N P S E K N S P S T Q Y C Y S I Q |
| GST-CD20 M1 | C E P A N P S E K N S P S T Q Y C G S I Q |
| GST-CD20 M2 | C E P A N P S E K N S P S T Q G C G S I Q |
| GST-CD20 M3 | C E P G N G S E K N S P S T Q Y C Y S I Q |
| GST-CD20 M4 | C E P A N P S G K N S P S T Q Y C Y S I Q |
Clone designation . | Sequence of CD20 homolog fused to GST . |
|---|---|
| GST-CD20 | C E P A N P S E K N S P S T Q Y C Y S I Q |
| GST-CD20 M1 | C E P A N P S E K N S P S T Q Y C G S I Q |
| GST-CD20 M2 | C E P A N P S E K N S P S T Q G C G S I Q |
| GST-CD20 M3 | C E P G N G S E K N S P S T Q Y C Y S I Q |
| GST-CD20 M4 | C E P A N P S G K N S P S T Q Y C Y S I Q |
Proteins with a mutation of the CD20 sequence are designated M1-M4. The amino acids considered to be important for rituximab binding were replaced by glycine (underlined letters). M4 had a mutation in a region adjacent but outside the ANPS peptide string, which is suspected to be critical for binding of rituximab.