General characteristics of heavy chain and light chain rearrangements in IGHV3-21 CLLs
| CLL case . | IGHV-D-J genes . | % mutation* . | Similar HCDR3, y/n† . | IGLV-J and IGKV-J genes . | % mutation‡ . | Similar L(K)CDR3, y/n† . | |||
|---|---|---|---|---|---|---|---|---|---|
| IGHV . | IGHD . | IGHJ . | IGL(K)V . | IGL(K)J . | |||||
| Mo66_N§ | 3-21 | 1-1 | 6 | 0.6 | y | IGLV3-21 | IGLJ3 | 2.0 | y |
| Mo107_N | 3-21 | 1-26 | 6 | 1.2 | y | IGLV3-21 & IGLV1-51 | IGLJ3 & IGLJ1 | 0.4 & 3.4 | y & n |
| B15_N‖ | 3-21 | 5-18 | 6 | 1.4 | y | IGLV3-21 & IGLV1-40 | IGLJ3 & IGLJ1 | 1.6 & 2.9 | y & n |
| No4937_N‖ | 3-21 | na | 6 | 1.5 | y | IGLV3-21 | IGLJ3 | 0.0 | y |
| B281_N | 3-21 | 5-18 | 6 | 2.1 | y | IGLV3-21 & IGLVI-40 | IGLJ3 & IGLJ2 | 1.9 & 4.1 | y & n |
| Mo91_N§ | 3-21 | 1-26 | 6 | 2.3 | y | IGLV3-21 & IGLV4-3 | IGLJ3 & IGLJ1 | 1.2 & 2.9 | y & n |
| No460_N | 3-21 | na | 6 | 2.4 | y | IGLV3-21 | IGLJ3 | 1.7 | y |
| Mo124_N§ | 3-21 | na | 6 | 2.9 | y | IGLV3-21 & IGLV3-21 | IGLJ3 & IGLJ1 | 1.2 & 1.2 | y & n |
| Mo145_N | 3-21 | na | 6 | 3.0 | y | IGLV3-21 & IGLV1-51 | IGLJ3 & IGLJ3 | 1.2 & 5.3 | y & n |
| Mo105_N§ | 3-21 | na | 6 | 3.4 | y | IGLV3-21 | IGLJ3 | 0.4 | y |
| B304_N‖ | 3-21 | 1-14 | 6 | 3.5 | y | IGLV3-21 & IGLV1-51 | IGLJ3 & IGLJ1 | 2.4 & 4.8 | y & n |
| B189_N‖ | 3-21 | na | 6 | 3.8 | y | IGLV3-21 | IGLJ3 | 2.6 | y |
| No4844_N‖ | 3-21 | 5-24 | 6 | 3.9 | y | IGLV3-21 | IGLJ3 | 2.1 | y |
| Mo2_N§ | 3-21 | na | 6 | 4.0 | y | IGLV3-21 & IGLV2-8 | IGLJ3 & IGLJ3 | 1.7 & 2.6 | y & n |
| Mo50_N§ | 3-21 | na | 6 | 4.2 | y | IGLV3-21 & IGLV6-57 | IGLJ3 & IGLJ1 | 1.6 & 4.0 | y & n |
| B164_N‖ | 3-21 | 5-24 | 6 | 4.4 | y | IGLV3-21 | IGLJ3 | 1.5 | y |
| B205_CS‖ | 3-21 | 5-24 | 6 | 0.4 | y | IGLV3-21 | IGLJ3 | 1.9 | y |
| B292_CS | 3-21 | 5-24 | 6 | 1.4 | y | IGLV3-21 | IGLJ3 | 2.0 | y |
| No1139_N | 3-21 | 3-10 | 6 | 0.0 | n | IGKV2-30 | IGKJ2 | 0.0 | n |
| Mo1_N§ | 3-21 | 3-3 | 5 | 0.0 | n | IGKV2-28 & IGKV3-20 | IGKJ2 & IGKJ2 | 0.0 & 2.9 | n |
| Mo28_N§ | 3-21 | 3-10 | 4 | 0.0 | n | IGLV3-12 | IGLJ3 | 0.8 | n |
| Mo37_N§ | 3-21 | 6-13 | 4 | 1.2 | n | IGLV9-49 | IGLJ3 | 0.0 | n |
| B110_N‖ | 3-21 | 3-22 | 4 | 3.0 | n | IGKV1-39 | IGKJ1 | 0.6 | n |
| Mo158_N | 3-21 | 6-13 | 6 | 4.8 | n | IGKV4-1 | IGKJ1 | 1.3 | n |
| B193_N‖ | 3-21 | 4-17 | 4 | 7.0 | n | IGLV3-21 | IGLJ3 | 2.3 | y |
| S3827_CS | 3-21 | 3-22 | 3 | 0.0 | n | IGLV3-21 | IGLJ3 | 0.0 | y |
| S5359_CS | 3-21 | 3-10 | 4 | 0.7 | n | IGKV4-1 | IGKJ3 | 0.0 | n |
| B338_CS‖ | 3-21 | 3-3 | 5 | 2.5 | n | IGKV1D-8 | IGKJ5 | 2.9 | n |
| G124_CS | 3-21 | 7-27 | 4 | 3.8 | n | na | na | na | na |
| B261_CS | 3-21 | 6-13 | 4 | 4.4 | n | IGKV1-8 | IGKJ4 | 1.2 | n |
| S3667_CS | 3-21 | 6-19 | 3 | 4.4 | n | IGKV1D-8 | IGKJ1 | 2.1 | n |
| S5153_CS | 3-21 | 3-22 | 6 | 4.4 | n | IGKV1-17 | IGKJ2 | 1.4 | n |
| B392_CS‖ | 3-21 | 1-26 | 3 | 4.7 | n | IGKV1-8 | IGKJ1 | 1.5 | n |
| S5427_CS | 3-21 | 3-22 | 4 | 5.1 | n | na | na | na | na |
| Si45_CS‖ | 3-21 | 6-13 | 2 | 6.5 | n | IGLV2-14 | IGLJ2 | 3.3 | n |
| G125_CS | 3-21 | 6-6 | 4 | 6.8 | n | na | na | na | na |
| B305_CS‖ | 3-21 | na | 5 | 7.1 | n | IGKV1-12 | IGKJ5 | 6.0 | n |
| CLL case . | IGHV-D-J genes . | % mutation* . | Similar HCDR3, y/n† . | IGLV-J and IGKV-J genes . | % mutation‡ . | Similar L(K)CDR3, y/n† . | |||
|---|---|---|---|---|---|---|---|---|---|
| IGHV . | IGHD . | IGHJ . | IGL(K)V . | IGL(K)J . | |||||
| Mo66_N§ | 3-21 | 1-1 | 6 | 0.6 | y | IGLV3-21 | IGLJ3 | 2.0 | y |
| Mo107_N | 3-21 | 1-26 | 6 | 1.2 | y | IGLV3-21 & IGLV1-51 | IGLJ3 & IGLJ1 | 0.4 & 3.4 | y & n |
| B15_N‖ | 3-21 | 5-18 | 6 | 1.4 | y | IGLV3-21 & IGLV1-40 | IGLJ3 & IGLJ1 | 1.6 & 2.9 | y & n |
| No4937_N‖ | 3-21 | na | 6 | 1.5 | y | IGLV3-21 | IGLJ3 | 0.0 | y |
| B281_N | 3-21 | 5-18 | 6 | 2.1 | y | IGLV3-21 & IGLVI-40 | IGLJ3 & IGLJ2 | 1.9 & 4.1 | y & n |
| Mo91_N§ | 3-21 | 1-26 | 6 | 2.3 | y | IGLV3-21 & IGLV4-3 | IGLJ3 & IGLJ1 | 1.2 & 2.9 | y & n |
| No460_N | 3-21 | na | 6 | 2.4 | y | IGLV3-21 | IGLJ3 | 1.7 | y |
| Mo124_N§ | 3-21 | na | 6 | 2.9 | y | IGLV3-21 & IGLV3-21 | IGLJ3 & IGLJ1 | 1.2 & 1.2 | y & n |
| Mo145_N | 3-21 | na | 6 | 3.0 | y | IGLV3-21 & IGLV1-51 | IGLJ3 & IGLJ3 | 1.2 & 5.3 | y & n |
| Mo105_N§ | 3-21 | na | 6 | 3.4 | y | IGLV3-21 | IGLJ3 | 0.4 | y |
| B304_N‖ | 3-21 | 1-14 | 6 | 3.5 | y | IGLV3-21 & IGLV1-51 | IGLJ3 & IGLJ1 | 2.4 & 4.8 | y & n |
| B189_N‖ | 3-21 | na | 6 | 3.8 | y | IGLV3-21 | IGLJ3 | 2.6 | y |
| No4844_N‖ | 3-21 | 5-24 | 6 | 3.9 | y | IGLV3-21 | IGLJ3 | 2.1 | y |
| Mo2_N§ | 3-21 | na | 6 | 4.0 | y | IGLV3-21 & IGLV2-8 | IGLJ3 & IGLJ3 | 1.7 & 2.6 | y & n |
| Mo50_N§ | 3-21 | na | 6 | 4.2 | y | IGLV3-21 & IGLV6-57 | IGLJ3 & IGLJ1 | 1.6 & 4.0 | y & n |
| B164_N‖ | 3-21 | 5-24 | 6 | 4.4 | y | IGLV3-21 | IGLJ3 | 1.5 | y |
| B205_CS‖ | 3-21 | 5-24 | 6 | 0.4 | y | IGLV3-21 | IGLJ3 | 1.9 | y |
| B292_CS | 3-21 | 5-24 | 6 | 1.4 | y | IGLV3-21 | IGLJ3 | 2.0 | y |
| No1139_N | 3-21 | 3-10 | 6 | 0.0 | n | IGKV2-30 | IGKJ2 | 0.0 | n |
| Mo1_N§ | 3-21 | 3-3 | 5 | 0.0 | n | IGKV2-28 & IGKV3-20 | IGKJ2 & IGKJ2 | 0.0 & 2.9 | n |
| Mo28_N§ | 3-21 | 3-10 | 4 | 0.0 | n | IGLV3-12 | IGLJ3 | 0.8 | n |
| Mo37_N§ | 3-21 | 6-13 | 4 | 1.2 | n | IGLV9-49 | IGLJ3 | 0.0 | n |
| B110_N‖ | 3-21 | 3-22 | 4 | 3.0 | n | IGKV1-39 | IGKJ1 | 0.6 | n |
| Mo158_N | 3-21 | 6-13 | 6 | 4.8 | n | IGKV4-1 | IGKJ1 | 1.3 | n |
| B193_N‖ | 3-21 | 4-17 | 4 | 7.0 | n | IGLV3-21 | IGLJ3 | 2.3 | y |
| S3827_CS | 3-21 | 3-22 | 3 | 0.0 | n | IGLV3-21 | IGLJ3 | 0.0 | y |
| S5359_CS | 3-21 | 3-10 | 4 | 0.7 | n | IGKV4-1 | IGKJ3 | 0.0 | n |
| B338_CS‖ | 3-21 | 3-3 | 5 | 2.5 | n | IGKV1D-8 | IGKJ5 | 2.9 | n |
| G124_CS | 3-21 | 7-27 | 4 | 3.8 | n | na | na | na | na |
| B261_CS | 3-21 | 6-13 | 4 | 4.4 | n | IGKV1-8 | IGKJ4 | 1.2 | n |
| S3667_CS | 3-21 | 6-19 | 3 | 4.4 | n | IGKV1D-8 | IGKJ1 | 2.1 | n |
| S5153_CS | 3-21 | 3-22 | 6 | 4.4 | n | IGKV1-17 | IGKJ2 | 1.4 | n |
| B392_CS‖ | 3-21 | 1-26 | 3 | 4.7 | n | IGKV1-8 | IGKJ1 | 1.5 | n |
| S5427_CS | 3-21 | 3-22 | 4 | 5.1 | n | na | na | na | na |
| Si45_CS‖ | 3-21 | 6-13 | 2 | 6.5 | n | IGLV2-14 | IGLJ2 | 3.3 | n |
| G125_CS | 3-21 | 6-6 | 4 | 6.8 | n | na | na | na | na |
| B305_CS‖ | 3-21 | na | 5 | 7.1 | n | IGKV1-12 | IGKJ5 | 6.0 | n |
The heavy and light chain rearrangements were performed by using either reverse-transcribed total RNA or genomic DNA. Purified amplicons were either directly sequenced or sequenced upon insertion into plasmid vector and cloning. In the latter case, percent mutation is the mean value of the percent mutations found in each clone. Each case is identified by a unique patient number; letters reported after the underscore symbol identify the Northern (N) or Central Southern (CS) geographic areas.
na indicates not assignable.
Refers to IGHV mutational status.
Evidence (y) or not (n) of similar aa HCDR3 or L(K)CDR3 composition.
Refers to IGL(K)V mutational status.
With the only exclusion of light chain data of case Mo2, data reported for these cases are exactly as reported by Thorselius et al.17
Cases used for GEP experiments.