Ligation of ectopic RXRα increases the MFI of CD11b
| Experiment no. . | MFI CD11b* . | |||||
|---|---|---|---|---|---|---|
| Control . | RXRα . | |||||
| Vehicle . | LG100268 . | VD3 . | Vehicle . | LG100268 . | VD3 . | |
| 1† | 166 | 123 | 303 | 132 | 332 | 364 |
| 2 | 130 | 137 | 241 | 112 | 232 | 291 |
| 3 | 124 | 99 | 202 | 114 | 179 | 212 |
| 4 | 160 | 157 | 305 | 220 | 447 | 435 |
| 5 | 97 | 102 | 380 | 105 | 220 | 493 |
| Experiment no. . | MFI CD11b* . | |||||
|---|---|---|---|---|---|---|
| Control . | RXRα . | |||||
| Vehicle . | LG100268 . | VD3 . | Vehicle . | LG100268 . | VD3 . | |
| 1† | 166 | 123 | 303 | 132 | 332 | 364 |
| 2 | 130 | 137 | 241 | 112 | 232 | 291 |
| 3 | 124 | 99 | 202 | 114 | 179 | 212 |
| 4 | 160 | 157 | 305 | 220 | 447 | 435 |
| 5 | 97 | 102 | 380 | 105 | 220 | 493 |
CD34+ cells were transduced with empty control or RXRα-encoding vectors under progenitor expansion conditions. After transduction (48 hours), cells were replated in serum-free Mo specific cultures in the absence (Vehicle) or presence of LG100268 or VD3 as indicated.
GFP+ cells were analyzed by FACS for the MFI of CD11b.
Five independent cord blood donor experiments.