Analysis of genes involved in IFN-α/β signaling and possible differences in IFN response
| Gene name . | Affymetrix ID . | iDC avg* . | A/P† . | P . | mDC avg . | A/P . | P . | Fold change (mDC/iDC)‡ . |
|---|---|---|---|---|---|---|---|---|
| IFNAR1 | 204191_at | 124 (55–188) | A/P | .165 | 139 (73–150) | A/P | .133 | 1.1 |
| IFNAR2 | 204786_s_at | 126 (51–279) | P | .004 | 122 (57–208) | P | .003 | 1.0 |
| JAK1 | 201648_at | 1377 (1227–1821) | P | <.001 | 3782 (2799–4632) | P | <.001 | 2.7 |
| TYK2 | 205546_s_at | 491 (315–880) | P | .011 | 963 (605–1229) | P | .005 | 2.0 |
| STAT1 | 200887_s_at | 1306 (892–1597) | P | <.001 | 730 (641–908) | P | <.001 | 0.6 |
| STAT1 | 97935_3_at | 720 (370–924) | P | <.001 | 474 (310–616) | P | <.001 | 0.7 |
| STAT2 | 205170_at | 164 (132–185) | P | .003 | 156 (109–189) | P | .009 | 0.9 |
| STAT3 | 208991_at | 928 (670–1179) | P | <.001 | 2084 (1458–2467) | P | <.001 | 2.2 |
| STAT4 | 206118_at | 206 (126–297) | A/P | .092 | 430 (319–507) | P | .024 | 2.1 |
| STAT5A | 203010_at | 492 (343–675) | P | .037 | 994 (772–1143) | P | .006 | 2.0 |
| STAT5B | 205026_at | 110 (87–139) | P | .008 | 239 (205–285) | P | .002 | 2.2 |
| STAT6 | 201331_s_at | 1975 (1773–2188) | P | <.001 | 1424 (1252–1681) | P | <.001 | 0.7 |
| STAT6 | 201332_s_at | 368 (306–428) | P | .007 | 269 (183–302) | A/P | .044 | 0.7 |
| Gene name . | Affymetrix ID . | iDC avg* . | A/P† . | P . | mDC avg . | A/P . | P . | Fold change (mDC/iDC)‡ . |
|---|---|---|---|---|---|---|---|---|
| IFNAR1 | 204191_at | 124 (55–188) | A/P | .165 | 139 (73–150) | A/P | .133 | 1.1 |
| IFNAR2 | 204786_s_at | 126 (51–279) | P | .004 | 122 (57–208) | P | .003 | 1.0 |
| JAK1 | 201648_at | 1377 (1227–1821) | P | <.001 | 3782 (2799–4632) | P | <.001 | 2.7 |
| TYK2 | 205546_s_at | 491 (315–880) | P | .011 | 963 (605–1229) | P | .005 | 2.0 |
| STAT1 | 200887_s_at | 1306 (892–1597) | P | <.001 | 730 (641–908) | P | <.001 | 0.6 |
| STAT1 | 97935_3_at | 720 (370–924) | P | <.001 | 474 (310–616) | P | <.001 | 0.7 |
| STAT2 | 205170_at | 164 (132–185) | P | .003 | 156 (109–189) | P | .009 | 0.9 |
| STAT3 | 208991_at | 928 (670–1179) | P | <.001 | 2084 (1458–2467) | P | <.001 | 2.2 |
| STAT4 | 206118_at | 206 (126–297) | A/P | .092 | 430 (319–507) | P | .024 | 2.1 |
| STAT5A | 203010_at | 492 (343–675) | P | .037 | 994 (772–1143) | P | .006 | 2.0 |
| STAT5B | 205026_at | 110 (87–139) | P | .008 | 239 (205–285) | P | .002 | 2.2 |
| STAT6 | 201331_s_at | 1975 (1773–2188) | P | <.001 | 1424 (1252–1681) | P | <.001 | 0.7 |
| STAT6 | 201332_s_at | 368 (306–428) | P | .007 | 269 (183–302) | A/P | .044 | 0.7 |
RNA from iDCs and mDCs was analyzed by Affymetrix microarray U133A. Affymetrix data were compiled from 4 individual donors. Average relative mRNA expression for iDCs (iDC avg) and mDCs (mDC avg) are indicated with the range indicated in parentheses.
Determination of the absence or presence (A/P) of a gene transcript as well as the P values were calculated using Affymetrix statistical software MAS5.0
Fold change of average expression levels are expressed as a ratio of mDC avg/iDC avg.