Monoclonal antibodies and fluorochromes used to examine macaque lymphocytes by 4-color and 8-color flow cytometry
| . | FITC . | PE . | APC . | PerCP . | PE-Cy5 . | PE-TR . | PE-Cy7 . | APC-Cy7 . | Pac. Blue . |
|---|---|---|---|---|---|---|---|---|---|
| 4-color flow cytometry | |||||||||
| Sample 1 | CD3 | CD20 | CD4 | CD8 | NA | NA | NA | NA | NA |
| Sample 2 | CD95* | CD28 | CD4 | CD8 | NA | NA | NA | NA | NA |
| Sample 3 | CD25 | CD69 | CD4 | CD8 | NA | NA | NA | NA | NA |
| 8-color flow cytometry | |||||||||
| Sample 4 | CD45RA | CCR5 | CD28 | NA | CD95 | CD8 | CD4 | CD69 | CD3 |
| . | FITC . | PE . | APC . | PerCP . | PE-Cy5 . | PE-TR . | PE-Cy7 . | APC-Cy7 . | Pac. Blue . |
|---|---|---|---|---|---|---|---|---|---|
| 4-color flow cytometry | |||||||||
| Sample 1 | CD3 | CD20 | CD4 | CD8 | NA | NA | NA | NA | NA |
| Sample 2 | CD95* | CD28 | CD4 | CD8 | NA | NA | NA | NA | NA |
| Sample 3 | CD25 | CD69 | CD4 | CD8 | NA | NA | NA | NA | NA |
| 8-color flow cytometry | |||||||||
| Sample 4 | CD45RA | CCR5 | CD28 | NA | CD95 | CD8 | CD4 | CD69 | CD3 |
NA indicates not applicable.
CD95/CD28 analysis for distinguishing naive and memory T-cell phenotypes was only performed on macaques from Tulane National Primate Research Center (see Table 1).