IFN-γ production by standard cDC subset preparations
| . | IFN-γ production, pg/mL . | ||
|---|---|---|---|
| Unstimulated . | With IL-12 + IL-18 . | With IL-12 + IL-18 + CpG1668 . | |
| CD4−CD8− cDCs | 180 ± 150 | 3 900 ± 710 | 6 640 ± 2 300 |
| CD4+CD8− cDCs | 40 ± 10 | 240 ± 100 | 350 ± 110 |
| CD4−CD8+ cDCs | 60 ± 40 | 110 ± 30 | 80 ± 20 |
| NK cells | ND | 771 210 ± 47 620 | 1 210 870 ± 51 040 |
| . | IFN-γ production, pg/mL . | ||
|---|---|---|---|
| Unstimulated . | With IL-12 + IL-18 . | With IL-12 + IL-18 + CpG1668 . | |
| CD4−CD8− cDCs | 180 ± 150 | 3 900 ± 710 | 6 640 ± 2 300 |
| CD4+CD8− cDCs | 40 ± 10 | 240 ± 100 | 350 ± 110 |
| CD4−CD8+ cDCs | 60 ± 40 | 110 ± 30 | 80 ± 20 |
| NK cells | ND | 771 210 ± 47 620 | 1 210 870 ± 51 040 |
The cDC subsets were isolated by the standard procedure of Figure 1, without depletion of cells bearing NK cell markers. NK cells were sorted from a whole-spleen suspension as CD49b+ cells. IFN-γ production was from the supernatant of 105 cells cultured in 0.2 mL medium for 50 hours, as detailed in “Materials and methods.” Results from a single experiment with 5 replicate cultures plus or minus SD, typical of multiple experiments.
ND indicates not determined.