Cytokine expression profile of LV-transduced CD4+ T cells
| Vector . | IL-2, % . | IFN-γ, % . | IL-4, % . |
|---|---|---|---|
| No vector | 24.2 ± 7.0 | 8.8 ± 0.4 | 1.8 ± 0.5 |
| HΔ24/FΔ30 | 22.3 ± 7.0 | 10.0 ± 0.1 | 2.2 ± 0.9 |
| VSV-G | 27.5 ± 9.5 | 12.1 ± 0.8 | 1.5 ± 0.6 |
| Vector . | IL-2, % . | IFN-γ, % . | IL-4, % . |
|---|---|---|---|
| No vector | 24.2 ± 7.0 | 8.8 ± 0.4 | 1.8 ± 0.5 |
| HΔ24/FΔ30 | 22.3 ± 7.0 | 10.0 ± 0.1 | 2.2 ± 0.9 |
| VSV-G | 27.5 ± 9.5 | 12.1 ± 0.8 | 1.5 ± 0.6 |
Quiescent CD4+ T cells were transduced with HΔ24/FΔ30 or VSV-G displaying LVs or incubated without vector and subsequently stimulated with PMA and ionomycin for 4 hours. Afterward, cells were permeabilized and incubated with anti–IL-2, anti–IL-4, or anti–IFN-γ sPE-coupled mAbs. As a control, an isotype PE–coupled mAb was used. Cells were washed, fixed, and analyzed by flow cytometry. The average of 3 experiments is shown (mean ±SD).