Correlation between complement regulatory protein expression and susceptibility to c1F6-mediated CDC
| Cell lines . | Complement regulatory receptors* . | c1F6-mediated CDC (no. experiments)† . | ||
|---|---|---|---|---|
| CD46 . | CD55 . | CD59 . | ||
| IM-9 | ++ | ++ | ++ | 5 ± 1 (4) |
| Raji | + | + | + | 7 ± 0 (2) |
| Farage | ++ | + | +++ | 0 ± 0 (3) |
| WIL2-S | + | + | − | 56 ± 4 (6) |
| MHH-PREB-1 | ++ | − | − | 73 ± 5 (9) |
| L-428 | +++ | + | +++ | 1 (1)‡ |
| U266 | ++ | − | + | 23 (1)‡ |
| LP-1 | ++ | − | − | 64 ± 4 (4) |
| 786-0 | ++ | +++ | ++ | 0 (1)‡ |
| Caki-1 | ++ | +++ | ++ | 0 (1)‡ |
| Cell lines . | Complement regulatory receptors* . | c1F6-mediated CDC (no. experiments)† . | ||
|---|---|---|---|---|
| CD46 . | CD55 . | CD59 . | ||
| IM-9 | ++ | ++ | ++ | 5 ± 1 (4) |
| Raji | + | + | + | 7 ± 0 (2) |
| Farage | ++ | + | +++ | 0 ± 0 (3) |
| WIL2-S | + | + | − | 56 ± 4 (6) |
| MHH-PREB-1 | ++ | − | − | 73 ± 5 (9) |
| L-428 | +++ | + | +++ | 1 (1)‡ |
| U266 | ++ | − | + | 23 (1)‡ |
| LP-1 | ++ | − | − | 64 ± 4 (4) |
| 786-0 | ++ | +++ | ++ | 0 (1)‡ |
| Caki-1 | ++ | +++ | ++ | 0 (1)‡ |
−indicates ratio less than 2; +, 2 to 9; ++, 10 to 49; and +++, at least 50.
Complement regulatory protein (CRP) expression described as ratio of mean fluorescence intensity binding of FITC-anti-CRP antibody to background fluorescence from isotype control antibody.
Percent maximal cell lysis mediated by saturating concentrations of c1F6 in the presence of normal human serum. Mean ± standard error.
Single experiment with triplicate samples.