Primer pairs used for RT-PCR to detect cytokine mRNA expression
. | Sequences . | . | . | |
|---|---|---|---|---|
| Mouse genes . | Forward . | Reverse . | Expected product size, bp . | |
| IL-6 | 5′-TTC TTG GGA CTG ATG CTG GT-3′ | 5′-CCA GTT TGG TAG CAT CCA TC-3′ | 269 | |
| IL-10 | 5′-TGC TAT GCT GCC TGC TCT TA-3′ | 5′-GCT CCA CTG CCT TGC TCT TA-3′ | 405 | |
| TGF-β | 5′-ACT ACT ATG CTA AAG AGG TCA-3′ | 5′-CTG TAT TCC GTC TCC TTG GTT-3′ | 341 | |
| TNF-α | 5′-TAT GTC TCA GCC TCT TCT CA-3′ | 5′-ACT TGG TGG TTT GCT ACG AC-3′ | 212 | |
| VEGF | 5′-AAC TTT CTG CTC TCT TGG GT-3′ | 5′-GTT CTG TCT TTC TTT GGT CT-3′ | 404 | |
| MCP-1 | 5′-TCA CCT GCT GCT ACT CAT TC-3′ | 5′-TCA CAC TGG TCA CTC CTA CA-3′ | 342 | |
| MCP-5 | 5′-GAT TTC CAC ACT TCT ATG GC-3′ | 5′-CGT TCG AGA CGT CTT ATC CA-3′ | 240 | |
| RANTES | 5′-CCT CAC CAT CAT CCT CAC TG-3′ | 5′-TAG CTC ATC TCC AAA TAG TT-3′ | 255 | |
| β-actin | 5′-CCA ACC GTG AAA AGA TGA CC-3′ | 5′-CCA TAC CCA AGA AGG AAG GC-3′ | 468 | |
. | Sequences . | . | . | |
|---|---|---|---|---|
| Mouse genes . | Forward . | Reverse . | Expected product size, bp . | |
| IL-6 | 5′-TTC TTG GGA CTG ATG CTG GT-3′ | 5′-CCA GTT TGG TAG CAT CCA TC-3′ | 269 | |
| IL-10 | 5′-TGC TAT GCT GCC TGC TCT TA-3′ | 5′-GCT CCA CTG CCT TGC TCT TA-3′ | 405 | |
| TGF-β | 5′-ACT ACT ATG CTA AAG AGG TCA-3′ | 5′-CTG TAT TCC GTC TCC TTG GTT-3′ | 341 | |
| TNF-α | 5′-TAT GTC TCA GCC TCT TCT CA-3′ | 5′-ACT TGG TGG TTT GCT ACG AC-3′ | 212 | |
| VEGF | 5′-AAC TTT CTG CTC TCT TGG GT-3′ | 5′-GTT CTG TCT TTC TTT GGT CT-3′ | 404 | |
| MCP-1 | 5′-TCA CCT GCT GCT ACT CAT TC-3′ | 5′-TCA CAC TGG TCA CTC CTA CA-3′ | 342 | |
| MCP-5 | 5′-GAT TTC CAC ACT TCT ATG GC-3′ | 5′-CGT TCG AGA CGT CTT ATC CA-3′ | 240 | |
| RANTES | 5′-CCT CAC CAT CAT CCT CAC TG-3′ | 5′-TAG CTC ATC TCC AAA TAG TT-3′ | 255 | |
| β-actin | 5′-CCA ACC GTG AAA AGA TGA CC-3′ | 5′-CCA TAC CCA AGA AGG AAG GC-3′ | 468 | |