Primer sets used for PCR and/or sequencing reactions
| HAS1 primers . | Sequence 5′ to 3′ . |
|---|---|
| 5′ exon 3 | GGGGTCTGTGCTGATCCTGG |
| 3′ exon 3 | GCTTCCAGTTTTATCCCATC |
| 5′ intron 3 | CTTCCACTGTGTATCCTGCATC |
| 3′ intron 3 | AACTGCTGCAAGAGGTTATTCC |
| 5′ exon 4 | TGGGGTTGGAACTGGAGATG |
| 3′ exon 4 | CATGCACACACGCTAGGATA |
| 5′ intron 4a | GCTCAGCATGGGTTATGCTA |
| 3′ intron 4a | GTATCCCCGCAGCTTAAACA |
| 5′ intron 4b | TTGGGATAATCCAGGGGAAT |
| 3′ intron 4b | CAAGATGGGTGTGGTTGCTA |
| 5′ intron 4c | GGTAGCAACCACACCCATCT |
| 3′ intron 4c | AGGAATGAGGGCATCATCG |
| 5′ exon 5a | CTCGCCCCCGTGCAGGTACA |
| 3′ exon 5a | AGGCCCCCAAGCAGCAGCAGCGC |
| 5′ exon 3-exon 4* | ATGGGATAGGCTTGGAGTCA* |
| 3′ exon 3-exon 4* | CCCATCCAAAACCCACTGCA* |
| 5′ exon 4-intron 4 (a)* | GGAGACCAAGGTAGCACAGT* |
| 3′ exon 4-intron 4 (a)* | GTCTCTTGCCCTTCCTACTT* |
| 5′ exon 4-intron 4 (b)* | CAAGGGTGGTGGATAGGAAGTT* |
| 3′ exon 4-intron 4 (b)* | CCTCAGGCACTCCACTTAACAC* |
| 5′ intron 4-exon 5 (a)* | GGGCACGATCATGGCTCACT* |
| 3′ exon 4-intron 4 (a)* | GAGGTAGGGGGATCACTTGA* |
| 5′ intron 4-exon 5 (b)* | CCCCAGGGAGCACGCGATGA* |
| 3′ exon 4-intron 4 (b)* | GCACGGGGGCGAGGAATGAG* |
| 5′ minigene | CTTCCACCTTACAGGTCTGTGACT |
| 3′ minigene | CCACTCTGGTTCATGGTGACTA |
| 5′intron 4c (T) | GCAAATACTATCTACTGGTCCTAAGC |
| 3′intron 4c (T) | TGTGTAGAATAGGGTGGATAATGGT |
| M13R | CAGGAAACAGCTATGAC |
| T7 | TAATACGACTCACTATAGGG |
| HAS1 primers . | Sequence 5′ to 3′ . |
|---|---|
| 5′ exon 3 | GGGGTCTGTGCTGATCCTGG |
| 3′ exon 3 | GCTTCCAGTTTTATCCCATC |
| 5′ intron 3 | CTTCCACTGTGTATCCTGCATC |
| 3′ intron 3 | AACTGCTGCAAGAGGTTATTCC |
| 5′ exon 4 | TGGGGTTGGAACTGGAGATG |
| 3′ exon 4 | CATGCACACACGCTAGGATA |
| 5′ intron 4a | GCTCAGCATGGGTTATGCTA |
| 3′ intron 4a | GTATCCCCGCAGCTTAAACA |
| 5′ intron 4b | TTGGGATAATCCAGGGGAAT |
| 3′ intron 4b | CAAGATGGGTGTGGTTGCTA |
| 5′ intron 4c | GGTAGCAACCACACCCATCT |
| 3′ intron 4c | AGGAATGAGGGCATCATCG |
| 5′ exon 5a | CTCGCCCCCGTGCAGGTACA |
| 3′ exon 5a | AGGCCCCCAAGCAGCAGCAGCGC |
| 5′ exon 3-exon 4* | ATGGGATAGGCTTGGAGTCA* |
| 3′ exon 3-exon 4* | CCCATCCAAAACCCACTGCA* |
| 5′ exon 4-intron 4 (a)* | GGAGACCAAGGTAGCACAGT* |
| 3′ exon 4-intron 4 (a)* | GTCTCTTGCCCTTCCTACTT* |
| 5′ exon 4-intron 4 (b)* | CAAGGGTGGTGGATAGGAAGTT* |
| 3′ exon 4-intron 4 (b)* | CCTCAGGCACTCCACTTAACAC* |
| 5′ intron 4-exon 5 (a)* | GGGCACGATCATGGCTCACT* |
| 3′ exon 4-intron 4 (a)* | GAGGTAGGGGGATCACTTGA* |
| 5′ intron 4-exon 5 (b)* | CCCCAGGGAGCACGCGATGA* |
| 3′ exon 4-intron 4 (b)* | GCACGGGGGCGAGGAATGAG* |
| 5′ minigene | CTTCCACCTTACAGGTCTGTGACT |
| 3′ minigene | CCACTCTGGTTCATGGTGACTA |
| 5′intron 4c (T) | GCAAATACTATCTACTGGTCCTAAGC |
| 3′intron 4c (T) | TGTGTAGAATAGGGTGGATAATGGT |
| M13R | CAGGAAACAGCTATGAC |
| T7 | TAATACGACTCACTATAGGG |
Primer set intron 4c (T) was used to verify common motif sequences 1st T, 2nd T, and TTTA stretches. Primer sequences were used in sequencing reactions only.