Impact of increasing CTAP-III concentration on PF-4 inhibition of NAP-2 generation by MCs and neutrophils
Cells and CTAP-III added, nM . | NAP-2 formed, nM . | . | . | |
|---|---|---|---|---|
| . | Without PF-4 . | With PF-4 . | % inhibition . | |
| MCs | ||||
| 1000 | 792 ± 124 | 96 ± 136 | 88.3 ± 17.1 | |
| 3000 | 2168 ± 615 | 326 ± 149 | 85.5 ± 4.0 | |
| 9000 | 3883 ± 1147 | 525 ± 89 | 85.3 ± 6.2 | |
| 27 000 | 8230 ± 3740 | 1570 ± 1071 | 82.0 ± 4.8 | |
| Neutrophils | ||||
| 1000 | 795 ± 83 | 253 ± 219 | 66.1 ± 29.3 | |
| 3000 | 2088 ± 194 | 648 ± 446 | 69.3 ± 19.4 | |
| 9000 | 5098 ± 697 | 1572 ± 731 | 70.1 ± 10.7 | |
| 27 000 | 10 607 ± 3199 | 3928 ± 1160 | 62.6 ± 4.5 | |
Cells and CTAP-III added, nM . | NAP-2 formed, nM . | . | . | |
|---|---|---|---|---|
| . | Without PF-4 . | With PF-4 . | % inhibition . | |
| MCs | ||||
| 1000 | 792 ± 124 | 96 ± 136 | 88.3 ± 17.1 | |
| 3000 | 2168 ± 615 | 326 ± 149 | 85.5 ± 4.0 | |
| 9000 | 3883 ± 1147 | 525 ± 89 | 85.3 ± 6.2 | |
| 27 000 | 8230 ± 3740 | 1570 ± 1071 | 82.0 ± 4.8 | |
| Neutrophils | ||||
| 1000 | 795 ± 83 | 253 ± 219 | 66.1 ± 29.3 | |
| 3000 | 2088 ± 194 | 648 ± 446 | 69.3 ± 19.4 | |
| 9000 | 5098 ± 697 | 1572 ± 731 | 70.1 ± 10.7 | |
| 27 000 | 10 607 ± 3199 | 3928 ± 1160 | 62.6 ± 4.5 | |
Different amounts of CTAP-III were incubated with α-IgE-stimulated MCs or unstimulated neutrophils in the absence or presence of a constant dosage of PF-4 (4 μM) for 30 minutes at 37°C. Generated NAP-2 was quantified by Li-cor analysis as described. Data represent mean ± SD from 3 different experiments.