Proteins identified by MALDI peptide mapping and ESI-MS/MS peptide sequencing
| Protein no. . | Protein name . | Accession no. or NCBI GI no. . | Spot intensity ratio at 24/48 h . | No. peptides found by MALDI-MS/ % match . | Peptides identified by ESI-MS/MS . |
|---|---|---|---|---|---|
| 1 | eIF4B | 48146033 | 1.8/2.4 | 1/2 | LGNLPYDVTEESIK |
| 2 | Heterogeneous nuclear ribonucleoprotein H | 48145673 | 2.6/4.0 | 4/11 | PSGEAFVELESEDEVK |
| 3 | erbB-3-binding protein 1 | 5453842 | 2.1/4.2 | 10/36 | — |
| 4 | Heat shock 70-kDa protein 5 | 16507237 | 5.2/2.6 | 14/23 | — |
| 5 | Chaperonin-containing TCP1 | 5453603 | 3.0/3.5 | 13/36 | — |
| 6 | eIF5A | 33383425 | 255/6.5 | 4/28 | VHLVGIDIFTGK; YEDICPSTHNMDVPNIK; NDFQLIGIQDGYLSLLQDSGEVR |
| 7 | Nucleophosmin | 825671 | 0/8.1 | 3/16 | MTDQEAIQDLWQWR; DELHIVEAEAMNYEGSPIK |
| 8 | Vinculin | 24657579 | 12/15 | —/— | SLGEISALTSK; ELLPVLISAMK |
| 9 | Ran-binding protein 1 | 47939632 | 288/61 | 2/14 | TLEEDEEELFK; PQFEPIVSLPEQEIK |
| 10 | Nucleosomal-binding protein 1 | 13529140 | 92.4/5.3 | 1/5 | LSAMLVPVTPEVKPK |
| 11 | Rho GDP dissociation inhibitor | 54111256 | 22.9/3.2 | 3/21 | TLLGDGPVVTDPK; ATFMoxVGSYGPRPEEYEFLTPVEEAPK; LGDGPVVTDPK |
| 12 | erbB-3-binding protein 1 | 5453842 | 3.1/11.3 | 5/19 | ITSGPFEPDLYK; FTVLLMoxPNGPMoxR; SLVEASSSGVSVLSLCcamEK |
| 13 | Similar to tubulin β5 | 2661079 | 0.4/0.4 | 5/15 | FPGQLNADLR; AILVDLEPGTMoxDSVR; ISVYYNEATGGK; LTTPTYGDLNHLVSATMoxSGVTTCcamLR |
| 14 | Heat shock 70-kDa protein 5 | 165072373 | 0.08/0.05 | 3/7 | TFAPEEISAMVLTK; IINEPTAAAIAYGLDK; VTHAVVTVPAYFNDAQR |
| 15 | Mortalin-2 | 21040386 | 0.2/0.4 | 7/14 | — |
| 16 | Annexin 1 | 54696696 | 0.1/0.2 | 10/34 | — |
| 17 | Serine hydroxymethyl-transferase | 539602 | 0.005/0.005 | 3/9 | AALEALGSCLNNK; TGLIDYNQLALTAR; ISATSIFFESMPYK |
| 18 | Valosin-containing protein | 11095436 | 0.3/0.2 | 1/4 | LGDVISIQPCPDVK |
| 19 | erb-3–binding protein 1 | 5453842 | 0.2/0.3 | 7/21 | — |
| Protein no. . | Protein name . | Accession no. or NCBI GI no. . | Spot intensity ratio at 24/48 h . | No. peptides found by MALDI-MS/ % match . | Peptides identified by ESI-MS/MS . |
|---|---|---|---|---|---|
| 1 | eIF4B | 48146033 | 1.8/2.4 | 1/2 | LGNLPYDVTEESIK |
| 2 | Heterogeneous nuclear ribonucleoprotein H | 48145673 | 2.6/4.0 | 4/11 | PSGEAFVELESEDEVK |
| 3 | erbB-3-binding protein 1 | 5453842 | 2.1/4.2 | 10/36 | — |
| 4 | Heat shock 70-kDa protein 5 | 16507237 | 5.2/2.6 | 14/23 | — |
| 5 | Chaperonin-containing TCP1 | 5453603 | 3.0/3.5 | 13/36 | — |
| 6 | eIF5A | 33383425 | 255/6.5 | 4/28 | VHLVGIDIFTGK; YEDICPSTHNMDVPNIK; NDFQLIGIQDGYLSLLQDSGEVR |
| 7 | Nucleophosmin | 825671 | 0/8.1 | 3/16 | MTDQEAIQDLWQWR; DELHIVEAEAMNYEGSPIK |
| 8 | Vinculin | 24657579 | 12/15 | —/— | SLGEISALTSK; ELLPVLISAMK |
| 9 | Ran-binding protein 1 | 47939632 | 288/61 | 2/14 | TLEEDEEELFK; PQFEPIVSLPEQEIK |
| 10 | Nucleosomal-binding protein 1 | 13529140 | 92.4/5.3 | 1/5 | LSAMLVPVTPEVKPK |
| 11 | Rho GDP dissociation inhibitor | 54111256 | 22.9/3.2 | 3/21 | TLLGDGPVVTDPK; ATFMoxVGSYGPRPEEYEFLTPVEEAPK; LGDGPVVTDPK |
| 12 | erbB-3-binding protein 1 | 5453842 | 3.1/11.3 | 5/19 | ITSGPFEPDLYK; FTVLLMoxPNGPMoxR; SLVEASSSGVSVLSLCcamEK |
| 13 | Similar to tubulin β5 | 2661079 | 0.4/0.4 | 5/15 | FPGQLNADLR; AILVDLEPGTMoxDSVR; ISVYYNEATGGK; LTTPTYGDLNHLVSATMoxSGVTTCcamLR |
| 14 | Heat shock 70-kDa protein 5 | 165072373 | 0.08/0.05 | 3/7 | TFAPEEISAMVLTK; IINEPTAAAIAYGLDK; VTHAVVTVPAYFNDAQR |
| 15 | Mortalin-2 | 21040386 | 0.2/0.4 | 7/14 | — |
| 16 | Annexin 1 | 54696696 | 0.1/0.2 | 10/34 | — |
| 17 | Serine hydroxymethyl-transferase | 539602 | 0.005/0.005 | 3/9 | AALEALGSCLNNK; TGLIDYNQLALTAR; ISATSIFFESMPYK |
| 18 | Valosin-containing protein | 11095436 | 0.3/0.2 | 1/4 | LGDVISIQPCPDVK |
| 19 | erb-3–binding protein 1 | 5453842 | 0.2/0.3 | 7/21 | — |