Primers, PCR product sizes, and positive controls for FGFR PCR analysis
| Gene . | PCR size, bp . | Positive control . | Primer sequences . | |
|---|---|---|---|---|
| Forward . | Reverse . | |||
| FGFR1 IIIb | 320 | Brain | 5′-CTTGACGTCGTGGAACGATCT-3′ | 5′-CACGCAGACTGGTTAGCTTCAC-3′ |
| FGFR1 IIIc | 344 | Brain | 5′-CTTGACGTCGTGGAACGATCT-3′ | 5′-AGAACGGTCAACCATGCAGAG-3′ |
| FGFR2 IIIb | 216 | Lung | 5′-CCCATCCTCCAAGCTGGACTG-3′ | 5′-CAGAGCCAGCACTTCTGCATTG-3′ |
| FGFR2 IIIc | 330 | Lung | 5′-CCCATCCTCCAAGCTGGACTG-3′ | 5′-TCTCACAGGCGCTGGCAGAAC-3′ |
| FGFR3 IIIb | 336 | Lung | 5′-CAAGTTTGGCAGCATCCGGCAGAC-3′ | 5′-TCTCAGCCACGCCTATGAAATTGGTG-3′ |
| FGFR3 IIIc | 138 | Brain (E13) | 5′-GGCGCTAACACCACCGAC-3′ | 5′-TGGCAGCACCACCAGCCAC-3′ |
| FGFR4 | 243 | Liver | 5′-GTACCCTCGGACCGCGGCACATAC-3′ | 5′-GCCGAAGCTGCTGCCGTTGATG-3′ |
| GAPDH | 446 | NA | 5′-GCCACCCAGAAGACTGTGGATGGC-3′ | 5′-CATGTAGGCCATGAGGTCCACCAC-3′ |
| Gene . | PCR size, bp . | Positive control . | Primer sequences . | |
|---|---|---|---|---|
| Forward . | Reverse . | |||
| FGFR1 IIIb | 320 | Brain | 5′-CTTGACGTCGTGGAACGATCT-3′ | 5′-CACGCAGACTGGTTAGCTTCAC-3′ |
| FGFR1 IIIc | 344 | Brain | 5′-CTTGACGTCGTGGAACGATCT-3′ | 5′-AGAACGGTCAACCATGCAGAG-3′ |
| FGFR2 IIIb | 216 | Lung | 5′-CCCATCCTCCAAGCTGGACTG-3′ | 5′-CAGAGCCAGCACTTCTGCATTG-3′ |
| FGFR2 IIIc | 330 | Lung | 5′-CCCATCCTCCAAGCTGGACTG-3′ | 5′-TCTCACAGGCGCTGGCAGAAC-3′ |
| FGFR3 IIIb | 336 | Lung | 5′-CAAGTTTGGCAGCATCCGGCAGAC-3′ | 5′-TCTCAGCCACGCCTATGAAATTGGTG-3′ |
| FGFR3 IIIc | 138 | Brain (E13) | 5′-GGCGCTAACACCACCGAC-3′ | 5′-TGGCAGCACCACCAGCCAC-3′ |
| FGFR4 | 243 | Liver | 5′-GTACCCTCGGACCGCGGCACATAC-3′ | 5′-GCCGAAGCTGCTGCCGTTGATG-3′ |
| GAPDH | 446 | NA | 5′-GCCACCCAGAAGACTGTGGATGGC-3′ | 5′-CATGTAGGCCATGAGGTCCACCAC-3′ |
NA indicates not applicable.