Reverse transcription–polymerase chain reaction human primer sequences information
| . | Primer pair sequence . | . | . | . | |
|---|---|---|---|---|---|
| Primer . | Forward . | Reverse . | Annealing temperature, °C . | Product size, bp . | |
| β-actin | ACCATGGATGATGATATCGCC | GTGCCAGATTTTCTCCATGTC | 55 | 244 | |
| PPAR-γ2 | GCTGTTATGGGTGAAACTCT | ATAAGGTGGAGATGCAGGCT | 55 | 351 | |
| LPL | GTCCGTGGCTACCTGTCAT | AGCCCTTTCTCAAAGGCTTC | 55 | 717 | |
| Adipsin | GGTCACCCAAGCAACAAAGT | CCTCCTGCGTTCAAGTCATC | 55 | 271 | |
| OPN | CACATCGGAATGCTCATTGC | ATCACCTGTGCCATACCAGT | 50 | 663 | |
| Cbfa-1 | ACTGGGCCCTTTTTCAGA | GCGGAAGCATTCTGGAA | 50 | 317 | |
| ALP | CTGGTAGGCGATGTCCTTA | ACGTGGCTAAGAATGTCATC | 50 | 475 | |
| OC | CTCACACTCCTCGCCCTA | CCTGAAAGCCGATGTGGT | 50 | 262 | |
| Coll II | TTTCCCAGGTCAAGATGGTC | TCACCTGGTTTTCCACCTTC | 54 | 498 | |
| Aggrecan | TGAGGAGGGCTGGAACAAGTACC | GGAGGTGGTAATTGCAGGGAACA | 60 | 350 | |
| Coll X | GCCCAAGAGGTGCCCCTGGAATAC | CCTGAGAAAGAGGAGTGGACATAC | 57 | 703 | |
| . | Primer pair sequence . | . | . | . | |
|---|---|---|---|---|---|
| Primer . | Forward . | Reverse . | Annealing temperature, °C . | Product size, bp . | |
| β-actin | ACCATGGATGATGATATCGCC | GTGCCAGATTTTCTCCATGTC | 55 | 244 | |
| PPAR-γ2 | GCTGTTATGGGTGAAACTCT | ATAAGGTGGAGATGCAGGCT | 55 | 351 | |
| LPL | GTCCGTGGCTACCTGTCAT | AGCCCTTTCTCAAAGGCTTC | 55 | 717 | |
| Adipsin | GGTCACCCAAGCAACAAAGT | CCTCCTGCGTTCAAGTCATC | 55 | 271 | |
| OPN | CACATCGGAATGCTCATTGC | ATCACCTGTGCCATACCAGT | 50 | 663 | |
| Cbfa-1 | ACTGGGCCCTTTTTCAGA | GCGGAAGCATTCTGGAA | 50 | 317 | |
| ALP | CTGGTAGGCGATGTCCTTA | ACGTGGCTAAGAATGTCATC | 50 | 475 | |
| OC | CTCACACTCCTCGCCCTA | CCTGAAAGCCGATGTGGT | 50 | 262 | |
| Coll II | TTTCCCAGGTCAAGATGGTC | TCACCTGGTTTTCCACCTTC | 54 | 498 | |
| Aggrecan | TGAGGAGGGCTGGAACAAGTACC | GGAGGTGGTAATTGCAGGGAACA | 60 | 350 | |
| Coll X | GCCCAAGAGGTGCCCCTGGAATAC | CCTGAGAAAGAGGAGTGGACATAC | 57 | 703 | |
ALP indicates alkaline phosphatase; OC, osteocalcin.