DL1, but not Jag1, inhibits pDC development in both percentage and absolute cell number
Experiment no. . | Control . | . | OP9-DL1 . | . | . | OP9-Jag1 . | . | . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | Percentage . | Absolute cell no. . | Percentage . | Absolute cell no. . | DL1/control ratio . | Percentage . | Absolute cell no. . | Jag1/control ratio . | |||||
| 1 | 4.6 | 32 873 | 0.1 | 12 180 | 0.37 | 1.3 | 29 900 | 0.91 | |||||
| 2 | 6.1 | 50 184 | 0.8 | 29 970 | 0.60 | 2.3 | 108 000 | 2.15 | |||||
| 3 | 22.6 | 119 833 | 2.8 | 47 040 | 0.39 | 14.9 | 143 232 | 1.20 | |||||
| 4 | 4.1 | 20 237 | 0.3 | 7 194 | 0.36 | 2.4 | 30 114 | 1.49 | |||||
| 5 | 7.4 | 109 372 | 0.4 | 14 964 | 0.14 | 3.4 | 55 094 | 0.50 | |||||
| 6 | 11.1 | 497 941 | 1.5 | 165 241 | 0.33 | 7.6 | 657 040 | 1.32 | |||||
| 7 | 33.0 | 726 000 | 1.0 | 258 000 | 0.36 | — | — | — | |||||
| 8 | 19.2 | 951 328 | 0.4 | 41 533 | 0.04 | — | — | — | |||||
| 9 | 13.3 | 29 260 | 0.2 | 1 058 | 0.04 | — | — | — | |||||
Experiment no. . | Control . | . | OP9-DL1 . | . | . | OP9-Jag1 . | . | . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | Percentage . | Absolute cell no. . | Percentage . | Absolute cell no. . | DL1/control ratio . | Percentage . | Absolute cell no. . | Jag1/control ratio . | |||||
| 1 | 4.6 | 32 873 | 0.1 | 12 180 | 0.37 | 1.3 | 29 900 | 0.91 | |||||
| 2 | 6.1 | 50 184 | 0.8 | 29 970 | 0.60 | 2.3 | 108 000 | 2.15 | |||||
| 3 | 22.6 | 119 833 | 2.8 | 47 040 | 0.39 | 14.9 | 143 232 | 1.20 | |||||
| 4 | 4.1 | 20 237 | 0.3 | 7 194 | 0.36 | 2.4 | 30 114 | 1.49 | |||||
| 5 | 7.4 | 109 372 | 0.4 | 14 964 | 0.14 | 3.4 | 55 094 | 0.50 | |||||
| 6 | 11.1 | 497 941 | 1.5 | 165 241 | 0.33 | 7.6 | 657 040 | 1.32 | |||||
| 7 | 33.0 | 726 000 | 1.0 | 258 000 | 0.36 | — | — | — | |||||
| 8 | 19.2 | 951 328 | 0.4 | 41 533 | 0.04 | — | — | — | |||||
| 9 | 13.3 | 29 260 | 0.2 | 1 058 | 0.04 | — | — | — | |||||
The absolute cell numbers represent numbers of pDC output cells derived from 50 000 input CD34+CD38− progenitor cells. The ratios are representative for the relative increase or decrease of pDCs cocultured with DL1 compared with the control cocultured cells.