Table 4

Effect of 1-hour incubation with 1 μM reserpine on the levels of DA, NE, and E and of their metabolites in cultured CD4+CD25+ regulatory T cells

Cells, 10−12 mol/106 cells
Medium, 10−12 mol/mL
Cells + medium, 10−12 mol/106 cells
ControlReserpineControlReserpineControlReserpine
l-DOPA 0.68 ± 0.23 1.04 ± 0.76 2.07 ± 0.98 2.55 ± 1.58 2.75 ± 1.14 3.59 ± 1.47 
DA 33.50 ± 8.17 6.56 ± 9.23* 1.54 ± 1.73 52.98 ± 16.92* 35.05 ± 9.85 59.54 ± 21.92† 
    3-MT 48.48 ± 12.88 41.44 ± 5.58 203.82 ± 158.32 48.19 ± 31.83† 252.29 ± 147.60 89.64 ± 36.81† 
    DOPAC 0.44 ± 0.24 10.80 ± 15.73 0.94 ± 0.22 0.91 ± 0.41 1.37 ± 0.46 11.71 ± 16.11 
    HVA 2.00 ± 0.44 6.01 ± 7.02 5.53 ± 4.82 2.09 ± 2.53 7.53 ± 4.93 8.09 ± 6.21 
NE 21.09 ± 4.35 10.33 ± 14.82 3.54 ± 1.74 27.90 ± 14.19* 24.63 ± 5.69 38.23 ± 24.58 
    NM 2.18 ± 1.92 23.28 ± 28.57 2.23 ± 1.28 6.62 ± 2.98* 4.41 ± 3.17 29.90 ± 29.50 
    VMA 15.04 ± 15.83 43.71 ± 41.92 113.28 ± 138.68 72.48 ± 110.28 128.31 ± 154.41 116.19 ± 109.09 
    MHPG 7.33 ± 1.80 30.59 ± 3.44* 14.99 ± 7.10 219.36 ± 173.22 22.33 ± 5.30 249.95 ± 176.66† 
    DHPG 18.39 ± 22.35 90.54 ± 93.81 ND ND NA NA 
E 20.31 ± 4.19 7.56 ± 10.44† 1.95 ± 1.45 35.33 ± 13.12* 22.26 ± 5.15 42.89 ± 19.08† 
    MET 0.54 ± 0.08 10.64 ± 11.46 0.66 ± 0.50 0.75 ± 0.79 1.20 ± 0.58 11.40 ± 10.68 
Cells, 10−12 mol/106 cells
Medium, 10−12 mol/mL
Cells + medium, 10−12 mol/106 cells
ControlReserpineControlReserpineControlReserpine
l-DOPA 0.68 ± 0.23 1.04 ± 0.76 2.07 ± 0.98 2.55 ± 1.58 2.75 ± 1.14 3.59 ± 1.47 
DA 33.50 ± 8.17 6.56 ± 9.23* 1.54 ± 1.73 52.98 ± 16.92* 35.05 ± 9.85 59.54 ± 21.92† 
    3-MT 48.48 ± 12.88 41.44 ± 5.58 203.82 ± 158.32 48.19 ± 31.83† 252.29 ± 147.60 89.64 ± 36.81† 
    DOPAC 0.44 ± 0.24 10.80 ± 15.73 0.94 ± 0.22 0.91 ± 0.41 1.37 ± 0.46 11.71 ± 16.11 
    HVA 2.00 ± 0.44 6.01 ± 7.02 5.53 ± 4.82 2.09 ± 2.53 7.53 ± 4.93 8.09 ± 6.21 
NE 21.09 ± 4.35 10.33 ± 14.82 3.54 ± 1.74 27.90 ± 14.19* 24.63 ± 5.69 38.23 ± 24.58 
    NM 2.18 ± 1.92 23.28 ± 28.57 2.23 ± 1.28 6.62 ± 2.98* 4.41 ± 3.17 29.90 ± 29.50 
    VMA 15.04 ± 15.83 43.71 ± 41.92 113.28 ± 138.68 72.48 ± 110.28 128.31 ± 154.41 116.19 ± 109.09 
    MHPG 7.33 ± 1.80 30.59 ± 3.44* 14.99 ± 7.10 219.36 ± 173.22 22.33 ± 5.30 249.95 ± 176.66† 
    DHPG 18.39 ± 22.35 90.54 ± 93.81 ND ND NA NA 
E 20.31 ± 4.19 7.56 ± 10.44† 1.95 ± 1.45 35.33 ± 13.12* 22.26 ± 5.15 42.89 ± 19.08† 
    MET 0.54 ± 0.08 10.64 ± 11.46 0.66 ± 0.50 0.75 ± 0.79 1.20 ± 0.58 11.40 ± 10.68 

Data are mean ± SD of 4-6 separate experiments.

ND indicates not determinable; NA indicates not applicable.

*

P < .01 versus control.

†

P < .05 versus control.

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