Sequence analysis of TCRG rearrangements at diagnosis and after engraftment
| Patient no. and sample source . | . | . | Clonal rearrangement sequence . | . |
|---|---|---|---|---|
| 6 | ||||
| Biopsy | (TRGV9*01) | tgtgccttgtgggaggt. | tttc | gaattattataagaaactcttt(TRGJ2*01) |
| CD34+/CD7− NOD/SCID 2° | (TRGV9*01) | tgtgccttgtgggaggt. | tttc | gaattattataagaaactcttt (TRGJ2*01) |
| 14 | ||||
| Biopsy | (TRGV3*01) | tgtgccacctgggacag. | cccca | gaattattataagaaactcttt (TRGJ2*01) |
| CD34+/CD4− NOD/SCID 2° | (TRGV3*01) | tgtgccacctgggacag. | cccca | gaattattataagaaactcttt (TRGJ2*01) |
| Biopsy | (TRGV11*01) | tgtgcctgct........... | atgagg | ...........agaaactcttt (TRGJ2*01) |
| CD34+/CD7− NOD/SCID 2° | (TRGV11*01) | tgtgcctgct........... | atgagg | ...........agaaactcttt (TRGJ2*01) |
| 2 | ||||
| Biopsy | (TRGV3*01) | tgtgccacctgg...... | tgatagg | ...ttattataagaaactcttt (TRGJ2*01) |
| CD34+/CD4− NOD/SCID 1° | (TRGV3*01) | tgtgccacctgg...... | tgatagg | ...ttattataagaaactcttt (TRGJ2*01) |
| Biopsy | (TRGV9*01) | tgtgccttgtgggagg. | aga | .aattattataagaaactcttt (TRGJ2*01) |
| CD34+/CD4− NOD/SCID 1° | (TRGV9*01) | tgtgccttgtgggagg. | aaaccttcctattattataagaaactctttggcagtgtaatattctcttgtggattgggg | ...............actcttt (TRGJ2*01) |
| Biopsy | (SIL Y07540) | caatttctggctcac | ccccaccc | gttggttttcatttcttc (TAL AL135960) |
| CD34+/CD4− NOD/SCID 1° | Clonal rearrangement not present | |||
| 13 | ||||
| Biopsy | (TRGV2*01) | tgtgccacctg....... | accacattataaac | gaattattataagaaactcttt (TRGJ2*01) |
| CD34+/CD7− NOD/SCID 2° | (TRGV2*01) | Clonal rearrangement not present |
| Patient no. and sample source . | . | . | Clonal rearrangement sequence . | . |
|---|---|---|---|---|
| 6 | ||||
| Biopsy | (TRGV9*01) | tgtgccttgtgggaggt. | tttc | gaattattataagaaactcttt(TRGJ2*01) |
| CD34+/CD7− NOD/SCID 2° | (TRGV9*01) | tgtgccttgtgggaggt. | tttc | gaattattataagaaactcttt (TRGJ2*01) |
| 14 | ||||
| Biopsy | (TRGV3*01) | tgtgccacctgggacag. | cccca | gaattattataagaaactcttt (TRGJ2*01) |
| CD34+/CD4− NOD/SCID 2° | (TRGV3*01) | tgtgccacctgggacag. | cccca | gaattattataagaaactcttt (TRGJ2*01) |
| Biopsy | (TRGV11*01) | tgtgcctgct........... | atgagg | ...........agaaactcttt (TRGJ2*01) |
| CD34+/CD7− NOD/SCID 2° | (TRGV11*01) | tgtgcctgct........... | atgagg | ...........agaaactcttt (TRGJ2*01) |
| 2 | ||||
| Biopsy | (TRGV3*01) | tgtgccacctgg...... | tgatagg | ...ttattataagaaactcttt (TRGJ2*01) |
| CD34+/CD4− NOD/SCID 1° | (TRGV3*01) | tgtgccacctgg...... | tgatagg | ...ttattataagaaactcttt (TRGJ2*01) |
| Biopsy | (TRGV9*01) | tgtgccttgtgggagg. | aga | .aattattataagaaactcttt (TRGJ2*01) |
| CD34+/CD4− NOD/SCID 1° | (TRGV9*01) | tgtgccttgtgggagg. | aaaccttcctattattataagaaactctttggcagtgtaatattctcttgtggattgggg | ...............actcttt (TRGJ2*01) |
| Biopsy | (SIL Y07540) | caatttctggctcac | ccccaccc | gttggttttcatttcttc (TAL AL135960) |
| CD34+/CD4− NOD/SCID 1° | Clonal rearrangement not present | |||
| 13 | ||||
| Biopsy | (TRGV2*01) | tgtgccacctg....... | accacattataaac | gaattattataagaaactcttt (TRGJ2*01) |
| CD34+/CD7− NOD/SCID 2° | (TRGV2*01) | Clonal rearrangement not present |
Unique V and J segments or SIL-TAL genes identified are shown in parentheses. Randomly deleted bases are represented by full stops; randomly inserted bases (both N and P nucleotides) are shown in italics.
1° and 2° indicate primary and secondary NOD/SCID recipients, respectively.