Survival rate of mice receiving Bcr-Abl-transduced WT or SHP-2Δ/Δ yolk sac cells
| . | WT/vector . | WT/Bcr-Abl . | SHP-2Δ/Δ/vector . | SHP-2Δ/Δ/Bcr-Abl . |
|---|---|---|---|---|
| Recipient animals, no. | 5 | 5 | 5 | 5 |
| Surviving animals, no. | 4 | 1 | 4 | 4 |
| . | WT/vector . | WT/Bcr-Abl . | SHP-2Δ/Δ/vector . | SHP-2Δ/Δ/Bcr-Abl . |
|---|---|---|---|---|
| Recipient animals, no. | 5 | 5 | 5 | 5 |
| Surviving animals, no. | 4 | 1 | 4 | 4 |
Primary yolk sac cells were isolated and transduced with Bcr-Abl and the vector control as described in Figure 1E. Transduced cells (2×103, with approximately 50% transduction efficiencies after 2 rounds of retroviral transduction) were mixed with bone marrow cells (5×105) freshly harvested from C57BL/6 mice and then transplanted into each lethally irradiated C57BL/6 mouse as described in “Materials and methods.” Survival analysis was done 6 months after transplantation.