Solenopsin inhibition of PKB/Akt is competitive with ATP
| ATP concentration, μM . | Activity, % control ± SD . |
|---|---|
| 1 | 25.6 ± 9.6 |
| 5 | 48.4 ± 3.6 |
| 20 | 64.3 ± 5.5 |
| 100 | 75.4 ± 7.3 |
| ATP concentration, μM . | Activity, % control ± SD . |
|---|---|
| 1 | 25.6 ± 9.6 |
| 5 | 48.4 ± 3.6 |
| 20 | 64.3 ± 5.5 |
| 100 | 75.4 ± 7.3 |
Solenopsin A (10 mM) was tested for its ability to inhibit PKBα at a range of ATP concentrations. The results are presented as the percentage of activity remaining in the presence of solenopsin relative to the activity measured in the absence of solenopsin. Results are expressed as the average of duplicate determinations. Similar results were obtained in several other experiments.