Conserved element mutant summary
CE . | Sequence . | Mutation . |
|---|---|---|
| 1 | AAAAGGGGG | AAAAGGGcc |
| 2 | TGACATC | acACATC |
| 3 | CAGATGGCTG | gtGATGGCTG |
| 4 | TGCCCCACC | TGCCaaACC |
| 6 | GTGACACAACCCTGTTCCCTGTC | GacACACAACCCTGTTCCCTGTC |
| 7 | GTTCCCGCTTATCTC | GTTaaCGCTTATCTC |
| 9 | TTCTTGGAAATGAG | ggaTTGGAAATGAG |
| 10 | AGAGCAAAGGGGAGG | AGAGCAAAGccaAGG |
CE . | Sequence . | Mutation . |
|---|---|---|
| 1 | AAAAGGGGG | AAAAGGGcc |
| 2 | TGACATC | acACATC |
| 3 | CAGATGGCTG | gtGATGGCTG |
| 4 | TGCCCCACC | TGCCaaACC |
| 6 | GTGACACAACCCTGTTCCCTGTC | GacACACAACCCTGTTCCCTGTC |
| 7 | GTTCCCGCTTATCTC | GTTaaCGCTTATCTC |
| 9 | TTCTTGGAAATGAG | ggaTTGGAAATGAG |
| 10 | AGAGCAAAGGGGAGG | AGAGCAAAGccaAGG |
Wild-type sequences for conserved elements in the hepcidin promoter are listed. To disrupt putative transcription factor binding sites, mutations in conserved elements (lower-case letters) were introduced individually into the hepcidin 0.6 kb-luc (mini) plasmid by site-directed mutagenesis.