Relative affinities of initial BR3 binding clones
. | Phage IC50 (nM) . | . | IgG EC50 (nM) . | . | ||
|---|---|---|---|---|---|---|
| Clones . | mBR3 . | hBR3 . | mBR3 . | hBR3 . | ||
| CB1 | 1700 | > 5000 | 50 | > 2000 | ||
| CB1a | > 5000 | NB | 20 | NB | ||
| CB1b | > 5000 | NB | NB | NB | ||
| CB1c | > 5000 | NB | 200 | NB | ||
. | Phage IC50 (nM) . | . | IgG EC50 (nM) . | . | ||
|---|---|---|---|---|---|---|
| Clones . | mBR3 . | hBR3 . | mBR3 . | hBR3 . | ||
| CB1 | 1700 | > 5000 | 50 | > 2000 | ||
| CB1a | > 5000 | NB | 20 | NB | ||
| CB1b | > 5000 | NB | NB | NB | ||
| CB1c | > 5000 | NB | 200 | NB | ||
Relative affinities for mBR3 and hBR3 ECD measured by competition phage ELISA or direct binding as purified IgG proteins to BR3-coated wells.
NB indicates no detectable binding.