Genotyping methods
SNP (allele); dbSNP accession no. . | Primers used for PCR . | Genotyping method . |
|---|---|---|
| CYP2C19 | ||
| 681 G>A (*2); rs4244285 | Sense: 5′-CAGAGCTTGGCATATTGTATC-3′ | Restriction fragment length polymorphism (RFLP) with SmaI |
| Anti-sense: 5′-GTAAACACACAAAACTAGTCAATG-3′ | ||
| 636 G>A (*3); rs4986893 | Sense: 5′-AACATCAGGATTGTAAGCAC-3′ | RFLP with BamHI |
| Anti-sense: 5′-TCAGGGCTTGGTCAATATAG-3′ | ||
| 1 A>G(*4); rs28399504 | Sense: 5′-GCACACACACTTAATTAGCATGG-3′ | Direct sequencing |
| Anti-sense: 5′-TACCTTTTGCAAGCCACTGA-3′ | ||
| 1297 C>T (*5); — | Sense: 5′-TGCATATTCTGTCTGTGCCAGT-3′ | Direct sequencing |
| Anti-sense: 5′-AGCAGCCAGACCATCTGT-3′ | ||
| 395 G>A (*6); — | Sense: 5′-CCTGGGATCTCCCTCCTAGT-3′ | Direct sequencing |
| Anti-sense: 5′-AGGAGAGCAGTCCAGAAAGG-3′ | ||
| CYP3A5 | ||
| 6986 G>A (*3); rs776746 | Sense: 5′-GAGTGGCATAGGAGATACCCAC-3′ | Direct sequencing |
| Anti-sense: 5′-TCTAGTTCATTAGGGTGTGACACACA-3′ | ||
| CYP2B6 | ||
| 1459 C>T (*5); rs3211371 | Sense: 5′-TGAGAATCAGTGGAAGCCATAGA-3′ | RFLP with BglII |
| Anti-sense: 5′-TAATTTTCGATAATCTCACTCCTGC-3′ | ||
| CYP1A2 | ||
| -164 A>C (*1F); rs762551 | Sense: 5′-CCCAGAAGTGGAAACTGAGA-3′ | RFLP with ApaI |
| Anti-sense: 5′-GGGTTGAGATGGAGACATTC-3′ |
SNP (allele); dbSNP accession no. . | Primers used for PCR . | Genotyping method . |
|---|---|---|
| CYP2C19 | ||
| 681 G>A (*2); rs4244285 | Sense: 5′-CAGAGCTTGGCATATTGTATC-3′ | Restriction fragment length polymorphism (RFLP) with SmaI |
| Anti-sense: 5′-GTAAACACACAAAACTAGTCAATG-3′ | ||
| 636 G>A (*3); rs4986893 | Sense: 5′-AACATCAGGATTGTAAGCAC-3′ | RFLP with BamHI |
| Anti-sense: 5′-TCAGGGCTTGGTCAATATAG-3′ | ||
| 1 A>G(*4); rs28399504 | Sense: 5′-GCACACACACTTAATTAGCATGG-3′ | Direct sequencing |
| Anti-sense: 5′-TACCTTTTGCAAGCCACTGA-3′ | ||
| 1297 C>T (*5); — | Sense: 5′-TGCATATTCTGTCTGTGCCAGT-3′ | Direct sequencing |
| Anti-sense: 5′-AGCAGCCAGACCATCTGT-3′ | ||
| 395 G>A (*6); — | Sense: 5′-CCTGGGATCTCCCTCCTAGT-3′ | Direct sequencing |
| Anti-sense: 5′-AGGAGAGCAGTCCAGAAAGG-3′ | ||
| CYP3A5 | ||
| 6986 G>A (*3); rs776746 | Sense: 5′-GAGTGGCATAGGAGATACCCAC-3′ | Direct sequencing |
| Anti-sense: 5′-TCTAGTTCATTAGGGTGTGACACACA-3′ | ||
| CYP2B6 | ||
| 1459 C>T (*5); rs3211371 | Sense: 5′-TGAGAATCAGTGGAAGCCATAGA-3′ | RFLP with BglII |
| Anti-sense: 5′-TAATTTTCGATAATCTCACTCCTGC-3′ | ||
| CYP1A2 | ||
| -164 A>C (*1F); rs762551 | Sense: 5′-CCCAGAAGTGGAAACTGAGA-3′ | RFLP with ApaI |
| Anti-sense: 5′-GGGTTGAGATGGAGACATTC-3′ |
Direct sequencing was performed using the Big Dye terminator cycle sequencing kit and an automated DNA sequencer (ABI 3100, Applied Biosystems).